Summary
DNA Profiling Summary (A-Level 2nd Year)
- Course
- Institution
DNA Profiling summary notes
[Show more]
Seller
Follow
z1
Content preview
DNA Profiling/FingerprintingPrinciples:
Uses non-coding DNA (doesn’t code for proteins) because all humans have SAME
GENES (Coding DNA)
Alleles = genes (base sequence) that have mutated (changed) bring about variation.
Uses repetitive sequences in non-coding DNA which vary in number of repeat
sequences (length of DNA fragment) in each individual
GEL ELECTROPHORESIS separates these DNA fragments by length.
Each individual had unique DNA barcode (except identical twins)
Each person has non-coding DNA in known LOCI that contains repeated DNA
sequence
Number of repeats of sequence varies between each person for each locus
*CAGTCACAGTCACAGTCA* = variable number tandem repeats (VNTR’s)
Short repeat *CATCATCAT* = Short Tandem Repeats (STR’s)
Number of repeats in sequence (VNTR’s/STR’s) makes length different
Each person carries 2 alleles of each of these repeat sequences because they get 1
allele from mum + 1 from dad. (if present on locus of 1 chromosome; will be at the
same locus on homologous chromosome.
VNTR/STR’s cut using restriction endonucleases
Enzymes - Cut DNA at specific base sequences
Mixture of VNTR/STRs can be separated by length + charge by gel electrophoresis
(chromatography)
DNA = Acid so gives away H+ and leaves DNA molecule to be negative
Use electricity to separate DNA segments of different lengths, those negative strands
go to positive electrode
Results in DNA fingerprint
Shorter DNA fragments = move faster than long ones
EXAM SPEAK METHOD
Uses VNTR/STRs – regions of non-coding with variable base sequence repeats
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller z1. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $7.09. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
67474 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now