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Big idea 13 Wetenschap en technologie
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Designing of novel antigenic peptide cocktail for the detection of antibodies to HIV - 1/2 by ELISA
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Designing of novelantigenic peptide
cocktail for the
detection of
antibodies to HIV-
1/2 by ELISA
Big Idea 13 Naeyaert Justine 1
, Natuurwetenschappen 3
Inhoud
1. Lijst met afkortingen en symbolen.....................................................................................................4
2. Verklarende woordenlijst...................................................................................................................6
3. Lijst met tabellen................................................................................................................................6
4. Lijst met figuren..................................................................................................................................7
6. Inleiding..............................................................................................................................................8
7. Humaan Immunodeficiëntie Virus......................................................................................................8
7.1. Wat is HIV?..................................................................................................................................8
7.2. Oorzaken en gevolgen.................................................................................................................9
7.2.1. Hoe krijg je HIV?...................................................................................................................9
7.2.2. Gevolgen van HIV..................................................................................................................9
8. HIV opsporen....................................................................................................................................10
9. Nieuwe detectiemethode door ELISA-techniek................................................................................10
9.1. Het materiaal en de methodes..................................................................................................10
9.1.1. Het ontwikkelen van de cocktail.........................................................................................10
9.2. HPLC-zuivering en sequentieanalyse.........................................................................................10
9.2.1. De ELISA-techniek...............................................................................................................13
9.3. Resultaten..................................................................................................................................14
9.4. Conclusie...................................................................................................................................15
10. Referentielijst.................................................................................................................................16
11. Bijlage.............................................................................................................................................17
I. Lijst met afkortingen en symbolen.................................................................................................3
II. Verklarende woordenlijst...............................................................................................................4
III. Lijst met tabellen........................................................................................................................5
IV. Lijst met figuren..........................................................................................................................5
1. Inleiding......................................................................................................................................6
2. Humaan Immunodeficiëntie Virus..............................................................................................6
2.1. Wat is HIV?.........................................................................................................................6
2.2. Oorzaken en gevolgen........................................................................................................7
2.2.1. Hoe krijg je HIV?.........................................................................................................7
2.2.2. Gevolgen van HIV........................................................................................................7
3. HIV opsporen..............................................................................................................................7
4. Nieuwe detectiemethode door ELISA-techniek..........................................................................8
4.1. Het materiaal en de methodes...........................................................................................8
Big Idea 13 Naeyaert Justine 2
, Natuurwetenschappen 3
4.1.1. Het ontwikkelen van de cocktail.................................................................................8
4.2. HPLC-zuivering en sequentieanalyse..................................................................................8
4.2.1. De ELISA-techniek.....................................................................................................10
4.3. Resultaten.........................................................................................................................11
4.4. Conclusie..........................................................................................................................12
VI. Referentielijst...........................................................................................................................13
VII. Bijlage.......................................................................................................................................14
Big Idea 13 Naeyaert Justine 3
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