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MCB 2050 Molecular Biology of the Cell - W21 Practice Midterm exam questions and answers tips with Dr. Yankulov University of Guelph CA$18.14   Add to cart
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MCB 2050 Molecular Biology of the Cell - W21 Practice Midterm exam questions and answers tips with Dr. Yankulov University of Guelph
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MCB 2050 Molecular Biology of the Cell - W21 Practice Midterm exam questions and answers tips with Dr. Yankulov University of Guelph PART A 1. Which of the following is NOT an essential component of a cloning vector? a. A unique restriction endonuclease cleavage b. A dominant sele...

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  • October 18, 2024
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  • 2024/2025
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MCB 2050 Molecular Biology of the Cell - W21 Practice Midterm exam
questions and answers tips with Dr. Yankulov University of Guelph




PART A

1. Which of the following is NOT an essential component of a cloning vector?
a. A unique restriction endonuclease cleavage
b. A dominant selectable marker gene
c. An origin of replication
d. A Taq polymerase binding site

2. Methylation of DNA sequence is used how by bacteria?
a. Methylation of DNA can inhibit cell division
b. Methylation in the promoter increases transcription of a given gene
c. Methylation in the promoter decreases transcription of a given gene
d. Methylation of DNA can prevent digestion by their own restriction enzymes
e. Methylation of DNA can stimulate DNA replication

3. Southern blotting detects , northern blotting detects and western blotting detects
.
i. RNA using a DNA probe
ii. Protein using an antibody probe
iii. Protein using a DNA probe
iv. DNA using a DNA probe
v. DNA using an RNA probe

a. i, iv, ii
b. ii, v, iii
c. v, I, iii
d. iv, i, ii

4. RT-PCR is used to:
a. Analyze genomic DNA sequences
b. Determine post-translational protein modifications
c. Determine DNA sequences
d. Determine alternative splicing junctions
e. Quantitate mRNA levels in a given sample

5. A DNA sequencing reaction contains which of the following?

, a. Dideoxyribonucleoside triphosphates
b. Deoxyribonucleoside triphosphates
c. DNA polymerase
d. B and C
e. All of the above

6. What is positional cloning?
a. Polymorphic tandem repeats only a few nucleotides long
b. Mapping genes to chromosomes by genetic mapping and then to locations by physical mapping
c. Short cDNA sequences of expressed genes used to map genes
d. Overlapping sets of DNA sequence clones

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