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Behaviour of biomolecules Lecture notes: Spectroscopy (Fluorescence)

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BSc Biomedical sciences course at the University of Southampton Behaviour of biomolecules Lecture notes: Spectroscopy (Fluorescence)

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  • November 7, 2023
  • 29
  • 2017/2018
  • Lecture notes
  • Phillip williamson
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BIOL1021:
Behavior  of  Biomolecules:
Spectroscopy

Dr  Philip  T.F.  Williamson
P.T.Williamson@soton.ac.uk
Centre  for  Biological  Sciences

, ROYAL SOCIETY OF CHEMISTRY DATA BOOK
THE ELECTROMAGNETIC SPECTRUM

The  electromagnetic  spectrum
f / Hz /m
1020 3 x 10­12

1019
X ­ rays
X-­ray 1018
420 nm (4.20 x 10­7 m)
Spectroscopy 1017 3x 10­9
Violet


Far ultraviolet Blue 470 nm
1016
UV/Vis
1015 3 x 10­7 Ultraviolet Green 530 nm
Spectroscopy
1014 Yellow 580 nm
Rotational/Vibrational Infrared
Spectroscopy 1013 3 x 10­5 Orange 620 nm
Rotational Far infrared
1012
Spectroscopy Red
700 nm (7.00 x 10­7 m)
1011 3 x 10­3
Electron  Resonance Microwave
1010 3 x 10­2
(NMR/MRI)
109 3 x 10­1

108 3
Nuclear  Magnetic
Resonance 107 Radio
(NMR/MRI)
106 3 x 102

105 2

, Interaction  of  electromagnetic
radiation  with  molecules
Resonates  with
transition  dipole  moment  (TDM)

Generates  oscillating
electromagnetic
field/wave Field/Wave  attenuated
H
N
N H2


OH



O

Oscillating
dipole TDM  starts  to  oscillate  (gains  energy),  absorbing  energy  for
oscillating  field/wave




3

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