BIOL1021:
Behavior of Biomolecules:
Nuclear Magnetic Resonance
Spectroscopy
Dr Philip T.F. Williamson
P.T.Williamson@soton.ac.uk
Centre for Biological Sciences
, ROYAL SOCIETY OF CHEMISTRY DATA BOOK
THE ELECTROMAGNETIC SPECTRUM
The electromagnetic spectrum
f / Hz /m
1020 3 x 1012
1019
X rays
X-ray 1018
420 nm (4.20 x 107 m)
Spectroscopy 1017 3x 109
Violet
Far ultraviolet Blue 470 nm
1016
UV/Vis
1015 3 x 107 Ultraviolet Green 530 nm
Spectroscopy
1014 Yellow 580 nm
Rotational/Vibrational Infrared
Spectroscopy 1013 3 x 105 Orange 620 nm
Rotational Far infrared
1012
Spectroscopy Red
700 nm (7.00 x 107 m)
1011 3 x 103
Electron Resonance Microwave
1010 3 x 102
(NMR/MRI)
109 3 x 101
108 3
Nuclear Magnetic
Resonance 107 Radio
(NMR/MRI)
106 3 x 102
105 2
, Spin
Spin (I) is an intrinsic property of
some nuclei and electrons.
In absence of magnetic field
energy levels are degenerate
Apply field
In presence of applied field can B0
adopt 2I+1 energy levels
For example:
ΔEmag=-µ.B0
• 1H, 13C, 15N, 31P are I=1/2
• 2H, 14N, are I=1
• 11B, 23Na, 35Cl are I=3/2
3
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