Used to highlight the difference between collagen and other connective tissue
Collagen stains red
Other tissue stains yellow
Toluidine Blue
For staining of acidic tissues
Nucleic acids stain blue
Mucins and cartilage become purple
Alcian Blue
Acid mucins stain blue
Nuclei stain pink
Giemsa
Stains human cells purple
o Nuclei are stained purple
o Cytoplasm stained blue to pale pink
Stains bacterial cells pink
Reticulin
Employs the use of silver impregnation
Used to assess histopathology of the liver
Fibres are stained black
Pink background
, Nuclei can be counterstained red
Orcein
Used to identify inclusion bodies of viruses
Inclusion bodies are stained dark brown-purple
Sudan Black
Used to identify lipids and lipofuscins
Stains lipofuscins black
Masson’s Trichrome
Used to differentiate muscle, collagen fibres, fibrin and erythrocytes in connective tissue
Nuclei stains blackish blue
Muscle and fibrin stain red
Collagen stains green
Mallory’s Trichrome
Stain shows the difference between collagen and muscle fibres
Nuclei and muscle cells stain red
Collagen stains blue
Erythrocytes stain orange
PAS
PAS stains glycogen to look at membranes and mucosubstances
Nuclei stain blue
Glycogen and fungi stain magenta
Gomori’s Aldehyde Fuchsin Stain
Used to stain elastic fibres and the pancreas
Elastic fibres stain bluish purple
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