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MIP quiz 3 Questions with 100% Actual correct answers | verified | latest update | Graded A+ | Already Passed | Complete Solution £6.48   Add to cart
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MIP quiz 3 Questions with 100% Actual correct answers | verified | latest update | Graded A+ | Already Passed | Complete Solution

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MIP quiz 3 Questions with 100% Actual correct answers | verified | latest update | Graded A+ | Already Passed | Complete Solution

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  • June 19, 2024
  • 2
  • 2023/2024
  • Exam (elaborations)
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  • mip quiz 3 questions with 100 actual correct answ

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MIP quiz 3
If you want to clone Gene 1 into the puC19 plasmid so it is in the same orientation as the
promoter, which of the following enzymes could you use to do this? Note: your amp gene
must be complete.
Note: ScaI, and SmaI are blunt end digesters. KpnI is a sticky end digester. - correct
answer-Kpn and SmaI to cut the plasmid and KpnI and ScaI to cut gene 1

You have the following restriction enzymes with the indicated recognition sequence. The ^
indicates the site of cleavage. If you digest a plasmid with BamHI, can you ligate it to a gene
of interest that has been Restriction enzyme digested with BgIII?
BamHI
G^GATCC
CCTAG^G
BglII
A^GATCT
TCTAG^A - correct answer-yes

You have the following restriction enzymes with the indicated recognition sequence. The ^
indicates the site of cleavage. If you digest a plasmid with BcII, can you ligate it to a gene of
interest that has been Restriction enzyme digested with BgIII?
BglII
A^GATCT
TCTAG^A
BclI
T^GATCA
ACTAG^T - correct answer-Yes

You have the following restriction enzymes with the indicated recognition sequence. The ^
indicates the site of cleavage. If you digest a plasmid with Sma I, can you ligate it to a gene
of interest that has been restriction enzyme digested with Psp AI?
Sma I
CCC^GGG
GGG^CCC
PspAI
A^CCGGT
TGGCC^A - correct answer-No

You have the following restriction enzymes with the indicated recognition sequence. The ^
indicates the site of cleavage. If you digest a plasmid with SmaI, can you ligate it to a gene
of interest that has been restriction enzyme digested with Xma I?
Xma I
C^CCGGG
GGGCC^C
Sma I
CCC^GGG

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