The proper blood to broth ratio for blood cultures to reduce the antibacterial effect of serum in adults
is: - 1:10
the most appropriate method for collecting a urine specimen from a patient with an indwelling
catheter? - aspirate urine aseptically from the catheter tubing
which of the following groups of specimens would be acceptable for anaerobic culture? - pleural fluid,
brain abcess fluid
sodium polyanetholsulfonate (SPS) is used as an anticoagulant for blood cultures because it: - inactivates
neutrophils and components of serum complement
the optimal collection of a wound specimen for culture of anaerobic organisms is a: - syringe filled with
pus, obtained before administration of antibiotics
the most important variable in the recovery of organisms in adult patients with bacteremia (bacterial
sepsis) is: - the recommended volume of blood ordered
virus transport medium should contain agents that: - inhibit bacterial and fungal growth
a bronchoscopy sample with the request culture for Legionella is sent to the lab. the correct plating
protocol is: - culture on buffered charcoal yeast extract agar with antibiotics
a community hospital microbiology lab is processing significant numbers of stool cultures because of an
outbreak of diarrhea following heavy rains and flooding in the county. a media that should be
incorporated in the plating protocol is: - thiosulfate citrate bile salts sucrose for Vibrio spp.
a male urethral discharge specimen submitted for culture should be inoculated to: - chocolate and
modified Thayer-Martin agars
Which selective medium is used for the isolation of gram-positive microorganisms? - Columbia CNA with
5% sheep blood
Campylobacter jejuni isolation requires the fecal specimen be: - inoculated onto selective plating media
and incubated in reduced oxygen with added CO2 at 42C
which of the following specimen requests is acceptable? - urine culture for acid-fast bacilli
a CSF containing only 2 drops of CSF was collected by a lumbar puncture from a 25 year old male, and
submitted for stat gram stain and culture. The direct specimen gram stain was reported as many
neutrophils and no microorganisms seen. The remaining drop of CSF should be inoculated to: -
chocolate agar
a diabetic food swab from an 82 year old woman with recurrent infections is submitted for culture. The
gram stain reveals:
Many neutrophils no squamous cells
many gram-neg bacilli
Many gram-pos cocci in chains
Dr requests that all pathogens be worked up, in addition to sheep blood, chocolate and MacConkey, the
tech may also process a: - CNA agar plate
which of the following is the most appropriate specimen source and primary media selection? -
endocervical; chocolate, Martin Lewis
which of the following is the most appropriate organism and media combo? - Yersinia enterocolitica-CIN
agar
a gram stain from a hand wound swab:
moderate neutrophils, no squamous, moderat GP cocci in clusters, moderate GN bacilli
the appropriate media that will selectively isolate each org? - CNA, MacConkey
, an organism that must be incubated in a microaerophilic environment for optimal recovery is: - C. jejuni
Vibrio parahaemolyticus is best isolated from feces on: - TCBS
Media used to support growth of Legionella pneumophila should contain which of the following
additives? - charcoal and yeast extract
the best medium for Bordatella pertussis is: - Regan-Lowe agar
a medium that can be used to recover Fancisella tularensis is: - Buffered charcoal yeast extract agar
a 21 year old pt presents with pharyngitis. A throat swab is collected and submitted for anaerobic
culture. This specimen should be: - rejected as unacceptable
An antibiotic used to suppress or kill contaminating fungi in media is: - cyclohexamide
a vaginal/rectal swab is collected from a pregnant pt to screen for group B strep. The best medium to
inoculate the specimen to is: - LIM broth
when processing a pt specimen for gram stain and culture, the proper use of a bio safety cabinet
includes: - not disrupting the air curtain barrier by keeping air flow and exhaust grills unobstructed
a wound specimen grew 2 colony types on sheep blood agar, and a single, clear colony on MacConkey.
SBA was documented as: #1: swarming over entire plate, GNR. #2 GP cocci in clusters.
The best way to isolate colony #2 from #1 is to subculture: - colony #2 to CNA and or PEA agar
a differential medium that can be used as a primary isolation agar producing predictable colored
colonies that can be distinguished from other organism colony types describes: - chromagar
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