Biology 427, exam 2
transcription and
translation exam(45
questions with 100%
correct answers)
In prokaryotes, transcription starts when RNA polymerase
binds to the: - answer Promotor
The catalytic center of the eubacterial RNA polymerase
includes the subunit(s): - answer β and β′
Which of the following is relatively unimportant for the
recognition of the promoter by sigma factor? - answer The
sequence of the region between the -10 and -35 elements
In general, termination of transcription in bacteria -
answer requires transcription of the sequences signaling
termination.
In phage SPO1, a(n) ___________ produced by one gene
allows later expression of other genes. - answer sigma
factor
, The bacterial RNA polymerase ternary complex includes -
answer sigma factor, core enzyme, RNA, and DNA
Which statement best describes the role of the eubacterial
RNA polymerase sigma factor? - answer Sigma factor
increases the affinity of RNA polymerase for promoter
sequences.
What information about the interaction between a protein
and DNA does a DNA footprint convey? - answer binding
site of a protein on the DNA
The most common sigma factor in E. coli is - answer sigma
70
The process of sporulation in Bacillus subtilis involves
more than one type of sigma factor. T or F? - answer True
One major difference between bacterial and eukaryotic
RNA polymerase is that - answer eukaryotic RNA
polymerase cannot read DNA to find the promoter
sequence.
An enhancer _____________________ the gene it affects. -
answer can be very distant from
Generally, a gene is more likely to be active if - answer its
DNA is unmethylated
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