MIP 302 Exam 2 Questions with All Correct Answers
Why are capture ELISAs also called sandwich ELISAs - Answer-they are called sandwich ELISAs because the target antigen is "sandwiched" between the 2 antibodies
What two components are common to all ELISAs? - Answer-Every ELISA will have eithe...
MIP 302 Exam 2 Questions
with All Correct Answers
Why are capture ELISAs also called sandwich ELISAs - Answer-they are called
sandwich ELISAs because the target antigen is "sandwiched" between the 2 antibodies
What two components are common to all ELISAs? - Answer-Every ELISA will have
either a target antigen or a capture antibody bound to a solid matrix. It will also have a
reporter system.
How does blocking help to inhibit false positive results? - Answer-blocking inhibits false
positives by blocking all of the binding sites not taken up by the Ag or Ab so another Ab
or Ag doesn't bind
Why are primary and secondary antibodies used in the same ELISA test made in
different animal species? - Answer-So that the secondary antibody will recognize the
primary antibody as "not self" and bind to it.
Imagine that you are using an ELISA to test blood for the presence of the virus that
causes hep B. You would assay for the presence of viral _______ in the blood -
Answer-antigen
Imagine that you are using an ELISA to test blood to determine if a person has been
exposed to HIV. You would assay for the presence of _________ in the blood - Answer-
antibody
Three methods of genetic recombo - Answer-transformation, conjugation, and
transduction
transformation - Answer-when a bacteria takes up a piece of DNA and plasmid in the
environment
conjugation - Answer-In bacteria, the direct transfer of DNA between two cells that are
temporarily joined.
Transduction - Answer-when a bacteriophage infects a cell and some of the genetic
material is exchanged
antibiotic resistance - Answer-comes from either spontaneous mutations or genetic
recombo
, Which of the 2 e. coli strains was the donor culture? What determines which strain is the
donor? - Answer-HT-99 carries the genes for chloramphenicol resistance on a plasmid
and is F+. Being F+ makes it the donor because it has the fertility (F) factor
Virus - Answer-A tiny, nonliving particle that invades and then reproduces inside a living
cell.
Viral Characteristics - Answer-viruses are inert unless they are inside a living cell,
viruses are acellular composed only of nucleic acid and protein shell. They also produce
progeny virion inside a living host cell, possess genetic material that is passed from one
'generation' to another, they are also infectious agents
Bacteriophage replication steps - Answer-1. attachment of phage to host cell
2. penetration of the bacterial cell wall
3. Synthesis of phage nucleic acids and proteins
4. assembly of the phage particles
5. release of the phage particles
animal virus replication - Answer-1. attachment of the virus to the host cell
2. entry or uptake of the virus into the host cell
3. removal of the protein coat surrounding the virus
4. synthesis of viral nucleic acids and proteins
5. assembly of viral protein and nucleic to form progeny virions
6. release of the virions from the host cell
attachment - Answer-the virus attaches to the host cell by means of a very specific
receptor site. For this reason, viruses will infect only certain hosts, and often only certain
cells in the particular host.
Bacteriophage - Answer-viruses that only infect bacterial cells
entry - Answer-bacteriophages essentially 'inject' their genetic material through the
bacterial cell wall, while many animal viruses enter through endocytosis. Animal cells
degrade foreign agents in an acidic lysosome. Sometimes the virus 'tricks' the cell by
attaching to the receptor site for some compound utilized by the cell so the cell will not
degrade it thinking it is a useful compound.
Uncoating - Answer-Endocytosis into a lysosome does not harm the virus. It only strips
away the protein coat, allowing the viral nucleic acid to enter the cell and take over the
cell machinery.
Synthesis of Viral Nucleic Acids and Proteins - Answer-Virus uses the cell to make viral
proteins and nucleic acid. How depends on what type of DNA/RNA the virus has
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