MCB 2050 Molecular Biology of the Cell upcoming exam 2024 cheat sheet with Prof. K. Yankulov University of Guelph
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MCB 2050 Molecular Biology of the Cell
Institution
MCB 2050 Molecular Biology Of The Cell
MCB 2050 Molecular Biology of the Cell upcoming exam 2024 cheat sheet with Prof. K. Yankulov University of Guelph
I synthesize pre-mRNA, what polymerase am I?
a) DNA polymerase
b) RNA pol III
c) RNA pol II
d) RNA pol I
d) RNA pol I
__________ is darkly staining and densely packed chrom...
MCB 2050 Molecular Biology of the Cell upcoming exam
2024 cheat sheet with Prof. K. Yankulov University of
Guelph
I synthesize pre-mRNA, what polymerase am I?
a) DNA polymerase
b) RNA pol III
c) RNA pol II
d) RNA pol I
d) RNA pol I
__________ is darkly staining and densely packed chromatin which is transcriptionally __________.
a) euchromatin; inactive
b) euchromatin; active
c) heterochromatin; inactive
d) heterochromatin; active
c) heterochromatin; inactive
Where is the core promoter relative to the +1 site of a gene?
a) Elements of the core promoter are located within about 35 base pairs both upstream and
downstream of the +1 site
,b) The location of the core promoter is not related to the +1 site
c) It can be hundreds of base pairs upstream or downstream from the +1 site
d) The core promoter is centred at ~-30 relative to the +1 site
e) The core promoter is located several base pairs downstream of the +1 site
a) Elements of the core promoter are located within about 35 base pairs both upstream and
downstream of the +1 site
What promoter element would you expect to find upstream of a gene that is highly expressed?
a) Initiator box
b) CpG islands
c) TATA box
d) DPE
c) TATA box
What promoter element would you expect to find upstream of a gene that is poorly expressed?
a) BRE
b) CpG islands
c) TATA box
d) DPE
b) CpG islands
A subunit within the general transcription factor __________ binds the TATA box during transcriptional
activation.
,TFIID
Put the following steps in order:
_____ A preformed complex of TFIIF and RNA pol II bind
_____ The TBP subunit of TFIID binds the TATA box, bending DNA
_____ TFIIA and TFIIB bind
_____ TFIIE binds creating a docking site for TFIIH, which then binds
1. The TBP subunit of TFIID binds the TATA box, bending DNA
2. TFIIA and TFIIB bind
3. A preformed complex of TFIIF and RNA pol II bind
4. TFIIE binds creating a docking site for TFIIH, which then binds
RNA pol II and the general transcription factor __________ bind the promoter as a pre-formed complex
TFIIF
In order to use a promoter plasmid to study the regulatory elements of a gene of interest, the
_________ of your gene of interest is inserted into the reporter plasmid _________.
a) open reading frame; upstream of the reporter gene
b) exons; upstream of the plasmid reporter
c) coding region; downstream of the plasmid reporter
d) promoter region; upstream of the reporter gene
e) structural region; downstream of the reporter gene
d) promoter region; upstream of the reporter gene
, Which element within the eukaryotic promoter has the most significant impact of transcription when
deleted?
Relative promoter activity after deletion....
TATA box- 38%
-40 to -70 region- 95%
GC box- 14%
CAAT box- 50%
-110 to -130 region- 114%
GC box
Sex determination in Drosophila embryos and post transcriptional regulation of the sxl, tra, and dsx
transcripts are an example of:
a) Non-disjunction
b) Alternate splicing
c) Gene expression
d) Transcriptional regulation
b) Alternate splicing
Which of the following RNA molecule binds perfectly to mRNA leading to it's rapid degradation?
Question 2 options:
a) miRNA
b) siRNA
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