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MCB 2050 Molecular Biology of the Cell - W21 upcoming Midterm exam preparation material – Dr. Yankulov (via Respondus Lockdown Browser and Webcam) University of Guelph£11.09
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MCB 2050 Molecular Biology of the Cell - W21 upcoming Midterm exam preparation material – Dr. Yankulov (via Respondus Lockdown Browser and Webcam) University of Guelph
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Module
MCB 2050 Molecular Biology of the Cell - W21
Institution
MCB 2050 Molecular Biology Of The Cell - W21
MCB 2050 Midterm W21 – Dr. Yankulov via Respondus Lockdown Browser and Webcam
PART A – Questions from tutorial
Question 1. You are studying the genes involved in embryonal development. Which of the following techniques could be used to visualize the locations with high gene expression of a ...
MCB 2050 Molecular Biology of the Cell -
W21 upcoming Midterm exam
preparation material – Dr. Yankulov (via
Respondus Lockdown Browser and
Webcam) University of Guelph
, MCB 2050 Midterm W21 – Dr. Yankulov via Respondus Lockdown Browser and Webcam
PART A – Questions from tutorial
Question 1. You are studying the genes involved in embryonal development. Which of the
following techniques could be used to visualize the locations with high gene expression of a
particular gene in a whole mounted embryo?
a) Western blotting
b) Northern blotting
c) Southern blotting
d) Immunoprecipitation
e) RNA in situ hybridization
Question 2. Why is Taq DNA polymerase enzyme used in PCR?
a) It is more accurate at the high temperatures required for the PCR reaction
b) It is faster and more efficient at the high temperatures required for the PCR
c) It functions at all steps of the PCR reaction
d) It does not denature at the high temperatures required for PCR enabling continuous
cycling through each step
e) More than one of the above is correct
Question 3. When analyzing the result of reverse transcriptase quantitative PCR (RT-qPCR)
experiment, a low Ct value indicates i) levels of target ii) in the original
sample.
a) I) low; ii) protein
b) I) high; ii) DNA
c) I) high; ii) mRNA
d) I) low; ii) DNA
e) I) low; ii) mRNA
Question 4.
In this picture from tutorial 3 you see the mutational analysis of the promoter of CXCR7.
Different versions of the plasmid with regions of the promoter are mutated (A-D). The plasmids
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