Summary
Summary calculations
- Module
- Zoology
- Institution
- University Of Reading (UoR)
calculation questions to help with moles
[Show more]
Seller
Follow
Content preview
Question 1:You have access to a 10x stock of running buffer. You need to make 500ml of 1x running buffer.
How much of the 10x stock do you need?
How much water do you need?
Question 2:
You have allowed some cells to attach to a glass coverslip. You would like to stain the membrane
with a dye. The dye is at a concentration of 1mg/ml. You have been told that it works well at a final
concentration of 4ug/ml diluted in PBS
You have 7 coverslips to stain and 300 ul will cover the coverslip. You need to prepare enough
mixture to cover all the coverslips.
How much mixture will you need to prepare, account for pipetting error?
How much of the stock dye will you need to use?
Question 3:
You have purified a protein but at 9mg/ml it is too concentrated. You decide to perform a serial 1:10
dilution. Determine the final concentration of the following
Neat
1:10
1:100
1:1000
Question 4:
Using your protein at 9mg/ml you perform a 1:3 standard curve. Determine the final concentration
of the following
Neat
1:3
1:9
1:27
1:81
1:243
Question 5:
To make 400 ul of a 1:250 dilution of antibody. How much antibody do you need to add?
Question 6:
You need to make the following lysis buffer
10 mM Tris-Cl (pH 8.0)
1 mM EDTA.
1% Triton X-100.
0.1% sodium deoxycholate.
0.1% SDS.
140 mM NaCl.
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller georgiawellmn. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for £2.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
60281 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy revision notes and other study material for 14 years now