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TSI (Triple Sugar Iron Agar)/ Micro Lab Tests Compilation Bundle
TSI (Triple Sugar Iron Agar)/ Micro Lab Tests Compilation Bundle
[Show more]TSI (Triple Sugar Iron Agar)/ Micro Lab Tests Compilation Bundle
[Show more]Is TSI gram+ or gram- correct answer: gram- 
 
What is the application of TSI? correct answer: used to differentiate members of Enterobacteriacea and to distinguish them from other gram- rods 
 
What is the media in TSI (triple sugar iron) correct answer: 1 glucose, lactose & sucrose 2 animal pro...
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Add to cartIs TSI gram+ or gram- correct answer: gram- 
 
What is the application of TSI? correct answer: used to differentiate members of Enterobacteriacea and to distinguish them from other gram- rods 
 
What is the media in TSI (triple sugar iron) correct answer: 1 glucose, lactose & sucrose 2 animal pro...
List the tests that are involved with TSI. correct answer: H2S Gas and Carbohydrate Fermentation 
 
What is the media for H2S Gas? correct answer: TSI agar 
 
What method do you use to place content on the media? correct answer: stab and drag motion 
 
what is the substrate for H2S gas? correct a...
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Add to cartList the tests that are involved with TSI. correct answer: H2S Gas and Carbohydrate Fermentation 
 
What is the media for H2S Gas? correct answer: TSI agar 
 
What method do you use to place content on the media? correct answer: stab and drag motion 
 
what is the substrate for H2S gas? correct a...
What does TSI mean? correct answer: Triple Sugar Iron 
 
What is a TSI (Triple Sugar Iron) Test also referred to as? correct answer: Kligers Iron Test 
 
What does a TSI Test, test for? correct answer: Bacteria's ability to: 
-Ferment glucose and lactose/sucrose 
-Produce gas 
-Reduce Sulfur 
 
...
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Add to cartWhat does TSI mean? correct answer: Triple Sugar Iron 
 
What is a TSI (Triple Sugar Iron) Test also referred to as? correct answer: Kligers Iron Test 
 
What does a TSI Test, test for? correct answer: Bacteria's ability to: 
-Ferment glucose and lactose/sucrose 
-Produce gas 
-Reduce Sulfur 
 
...
For what bacteria would you use the TSI test? correct answer: Gram negative bacilli such as enterobacteriaceae. 
 
How would you identify an acidic slant? correct answer: If the slant goes from red to yellow on the phenol red. 
 
Why is there more lactose and sucrose in TSI agar than glucose? corr...
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Add to cartFor what bacteria would you use the TSI test? correct answer: Gram negative bacilli such as enterobacteriaceae. 
 
How would you identify an acidic slant? correct answer: If the slant goes from red to yellow on the phenol red. 
 
Why is there more lactose and sucrose in TSI agar than glucose? corr...
TSI stands for________________________? correct answer: Triple Sugar Iron 
 
What is the sulfur source in TSI agar? correct answer: Sodium thiosulfate 
 
A red slant and a red butt with TSI agar indicates that the microorganism ________? correct answer: Cannot ferment 
 
A red slant and a yellow ...
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Add to cartTSI stands for________________________? correct answer: Triple Sugar Iron 
 
What is the sulfur source in TSI agar? correct answer: Sodium thiosulfate 
 
A red slant and a red butt with TSI agar indicates that the microorganism ________? correct answer: Cannot ferment 
 
A red slant and a yellow ...
What sugars does triple sugar iron agar contain? correct answer: 1% Lactose 
1% Sucrose 
0.1% Glucose (Dextrose) 
 
What is TSI used for? correct answer: To differentiate enterics based on the ability to reduce sulfur and ferment carbohydrates 
 
What is fermented with a red (alkaline) slant and a...
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Add to cartWhat sugars does triple sugar iron agar contain? correct answer: 1% Lactose 
1% Sucrose 
0.1% Glucose (Dextrose) 
 
What is TSI used for? correct answer: To differentiate enterics based on the ability to reduce sulfur and ferment carbohydrates 
 
What is fermented with a red (alkaline) slant and a...
TSI stands for correct answer: Triple Sugar Iron 
 
If peptone is metabolized then what is made correct answer: Ammonia 
 
What are the 3 carbohydrates present for TSI correct answer: Glucose Sucrose Lactose 
 
Glucose is a correct answer: monosaccharide 
 
Sucrose and Lactose are correct answer...
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Add to cartTSI stands for correct answer: Triple Sugar Iron 
 
If peptone is metabolized then what is made correct answer: Ammonia 
 
What are the 3 carbohydrates present for TSI correct answer: Glucose Sucrose Lactose 
 
Glucose is a correct answer: monosaccharide 
 
Sucrose and Lactose are correct answer...
Phenylethyl alcohol agar PAA correct answer: Selective media- used to isolate specific groups of bacteria 
 
Isolation of mostly Gram positive organisms 
 
** Phenylethyl alcohol ** inhibits Gram negative organisms 
colonies may be visible , but smaller in number and size 
 
Phenylethyl alcohol a...
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Add to cartPhenylethyl alcohol agar PAA correct answer: Selective media- used to isolate specific groups of bacteria 
 
Isolation of mostly Gram positive organisms 
 
** Phenylethyl alcohol ** inhibits Gram negative organisms 
colonies may be visible , but smaller in number and size 
 
Phenylethyl alcohol a...
FeCl3 
Used in a PAA agar to detect presence of phenylpyruvic acid positive green color. 
 
MAC agar 
 
What stain do you do on Day 1 of the unknown lab? 
You may Gram stain from your unknown broth to confirm if the bacteria are Gram-positive or Gram-negative. 
A throat culture performed by using ...
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Add to cartFeCl3 
Used in a PAA agar to detect presence of phenylpyruvic acid positive green color. 
 
MAC agar 
 
What stain do you do on Day 1 of the unknown lab? 
You may Gram stain from your unknown broth to confirm if the bacteria are Gram-positive or Gram-negative. 
A throat culture performed by using ...
Organisms that require specialized environments due to complex nutritional requirements are known as...? correct answer: fastidious organisms 
 
The blood agar plate (BAP) is an enrichment medium that tests for fastidious organisms such as? correct answer: Streptococcus spp. (species) 
 
The blood...
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Add to cartOrganisms that require specialized environments due to complex nutritional requirements are known as...? correct answer: fastidious organisms 
 
The blood agar plate (BAP) is an enrichment medium that tests for fastidious organisms such as? correct answer: Streptococcus spp. (species) 
 
The blood...
TSI Agar correct answer: *stab and drag 
*yellow= acid production/ fermentaion of the sugars in the slant/butt. 
*red= negative 
*black= H2S production (salmonella or proteus) 
 
what are we looking for in the Indole test after adding kovacs reagant? correct answer: * a red ring which indicates th...
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Add to cartTSI Agar correct answer: *stab and drag 
*yellow= acid production/ fermentaion of the sugars in the slant/butt. 
*red= negative 
*black= H2S production (salmonella or proteus) 
 
what are we looking for in the Indole test after adding kovacs reagant? correct answer: * a red ring which indicates th...
*Selective:* 
Selects for: Gram (+) 
*Components:* 
Phenylethyl alcohol 
(used for isolation of Gram + from mixed cultures) 
 
Plate A is a PEA plate with a Gram positive organism on the left and a Gram negative organism on the right. Plate B is a nutrient agar plate illustrating normal growth of ea...
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Add to cart*Selective:* 
Selects for: Gram (+) 
*Components:* 
Phenylethyl alcohol 
(used for isolation of Gram + from mixed cultures) 
 
Plate A is a PEA plate with a Gram positive organism on the left and a Gram negative organism on the right. Plate B is a nutrient agar plate illustrating normal growth of ea...
Why is the catalytic activity of enzymes essential to ensure and regulate cellular metabolism? correct answer: enzymes lower the activation energy for chemical rxn. 
 
Why are microorganisms able to cause diary products, such as milk, to sour or curdle? correct answer: microorganisms can break dow...
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Add to cartWhy is the catalytic activity of enzymes essential to ensure and regulate cellular metabolism? correct answer: enzymes lower the activation energy for chemical rxn. 
 
Why are microorganisms able to cause diary products, such as milk, to sour or curdle? correct answer: microorganisms can break dow...
What does TSI stand for? correct answer: Triple Sugar Iron 
 
How do you inoculate a TSI? correct answer: Stab and Drag 
 
What does a TSI slant agar select for? correct answer: Differentiates between non-fermenters, glucose fermenters and fermenters of lactose and/or sucrose. It is used to ident...
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Add to cartWhat does TSI stand for? correct answer: Triple Sugar Iron 
 
How do you inoculate a TSI? correct answer: Stab and Drag 
 
What does a TSI slant agar select for? correct answer: Differentiates between non-fermenters, glucose fermenters and fermenters of lactose and/or sucrose. It is used to ident...
TSI correct answer: Triple Sugar Iron 
Yellow= acid production or fermentation of the sugars in the slant or butt. 
 
Gas production= indicated by the agar lifting up or breaking 
 
Red= negative or uninoculated 
 
Black= H2S production due to FeS precipitation (think salmonella or proteus) 
 
The ...
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Add to cartTSI correct answer: Triple Sugar Iron 
Yellow= acid production or fermentation of the sugars in the slant or butt. 
 
Gas production= indicated by the agar lifting up or breaking 
 
Red= negative or uninoculated 
 
Black= H2S production due to FeS precipitation (think salmonella or proteus) 
 
The ...
Foods associated with Salmonella outbreak correct answer: eggs, meat, poultry 
 
detection and isolation procedure for salmonella correct answer: pre-enrichment 
selective enrichment 
streak for isolation 
confirmation using slants 
further confirmation using PCR 
 
media used for pre-enrichment o...
Preview 2 out of 9 pages
Add to cartFoods associated with Salmonella outbreak correct answer: eggs, meat, poultry 
 
detection and isolation procedure for salmonella correct answer: pre-enrichment 
selective enrichment 
streak for isolation 
confirmation using slants 
further confirmation using PCR 
 
media used for pre-enrichment o...
Foods associated with Salmonella outbreak correct answer: eggs, meat, poultry 
 
detection and isolation procedure for salmonella correct answer: pre-enrichment 
selective enrichment 
streak for isolation 
confirmation using slants 
further confirmation using PCR 
 
media used for pre-enrichment o...
Preview 2 out of 9 pages
Add to cartFoods associated with Salmonella outbreak correct answer: eggs, meat, poultry 
 
detection and isolation procedure for salmonella correct answer: pre-enrichment 
selective enrichment 
streak for isolation 
confirmation using slants 
further confirmation using PCR 
 
media used for pre-enrichment o...
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