HISTOTECHNOLOGY TESTS BUNDLE SET

Periodic Acid Schiff stain demonstrates CORRECT ANSWER-Polysaccharides, neutral mucosubstances, and basement membranes Periodic acid and Chromic acid oxidise carbohydrates to CORRECT ANSWER-aldehydes What acid causes loss of quinoid structure in basic fuchsin? CORRECT ANSWER-Sulfurous acid ...

Acetic acid CORRECT ANSWER-This fixative does not fix or destroy carbohydrates, and it does not fix lipids. It penetrates very rapidly and leaves tissue very soft. It is added to many fixative mixtures because of its ability to fix nuclei. Glutaraldehyde CORRECT ANSWER-Techniques using Schiff rea...

fixitive used for specimens CORRECT ANSWER-10% neutral buffered Formalin Marking Margins CORRECT ANSWER-- to identify resection margins, if tumour too close to these margins they know they have to go in and cut more out - Guide to embedding What is used to clean grossing station CORRECT ANSWE...

What are the two common staining methods for nucleic acid in histology? CORRECT ANSWER-1. Feulgen Reaction 2. Methyl Green-Pyronin Method (DNA/RNA) Feulgen Reaction tests for what? CORRECT ANSWER-DNA What does the Schiff reagent bind to in the Feulgen Reaction? CORRECT ANSWER-Aldehyde group c...

Block Holder (Chuck) Knife & knife carrier Paul, ratchet feed wheel & adjustment screws CORRECT ANSWER-3 essential parts of microtome Block holder CORRECT ANSWER-Holds the tissue in position Knife and knife carrier CORRECT ANSWER-For actual cutting of tissue sections Pawl, ratchet feed wh...

What thickness are paraffin sections routinely cut? CORRECT ANSWER-4-6 um Why should the temperature of the flotation bath be set specifically at 5-10 °C below the melting point of the paraffin wax? CORRECT ANSWER-water bath that is too hot may distort tissue morphology Warm enough to reliev...

Place in a separate container CORRECT ANSWER-Match each listed item with the appropriate disposal method: Used silver nitrate Simply pour down the sink CORRECT ANSWER-Match each listed item with the appropriate disposal method: 1% alcohol Dilute first, pour down the sink CORRECT ANSWER-M...

What are the steps of processing? CORRECT ANSWER-Fixation, (decalcification), dehydration, clearing, infiltration, embedding The step of processing removes water for the next subsequent step to occur (xylene and paraffin) CORRECT ANSWER-dehydration What accounts for the majority of tissue proc...

affinity or avidity:a dye is attracted to a tissue CORRECT ANSWER-affinity affinity or avidity: how strongly a dye is attracted to a tissue CORRECT ANSWER-avidity what happens when amphoteric tissue (or dye) is placed in an acidic solvent? CORRECT ANSWER-becomes basic what happens when amph...

What is the purpose of embedding? CORRECT ANSWER-hold tissue; orient tissue Why do we want the tissue held? CORRECT ANSWER-so we can cut very thin sections and it has more stability Why do we orient tissue? CORRECT ANSWER-so that the view the pathologist has is optimal for diagnosis List th...

formalin CORRECT ANSWER-Which of the following pigments is birefringent? A. formalin B. bile C. lipofuscin D. melanin 8 - 10 micrometer CORRECT ANSWER-What micrometer thickness is preferred for the technique shown? skeletal CORRECT ANSWER-Muscle that histologically contains cytoplasmic cro...

An example of an additive fixative is one that contains: CORRECT ANSWER-picric acid Microscopic examination of an H&E stained section fixed in formalin shows marked nuclear bubbling. One most often sees this artifact if the specimen is processed following: CORRECT ANSWER-incomplete fixation Mi...

For adequate fixation, the fixative volume should be at least ______ times greater than the tissue volume. A. Equal B. 5-10 C. 10-14 D. 15-20 CORRECT ANSWER-D. 15-20 This type of microscope is used for the identification of crystals as well as to make identification of amyloid. A. Light...

T/F To be characterized as a profession, considerable specialized training must be required. CORRECT ANSWER-T Histology is the study of CORRECT ANSWER-tissue T/F The purpose of having a procedure for specimen receipt is to monitor the specimen and the accompanying paperwork. CORRECT ANSWER-T ...