BCMB 406B - Lab exam questions and solutions bundle!!

Glycoside hydrolases - Hydrolyze the glycosidic bond between 2 or more carbohydrates or carbohydrate-protein bonds Carbohydrate Binding Modules - - Amino acid sequence within a carbohydrate-active enzyme with a discreet fold that has carbohydrate binding activity - Maintains the interaction of t...

The success of a PCR reaction is mostly dependent on what? - well designed primers Primer design can also be applied to what? - - probes in Southern and Northern blots - DNA sequencing - microarray design What else can affect the success of a PCR reaction? - - Quality of the template DNA - cy...

what variables are considered in primer design - length, sequence, %GC, Tm, Ta, 3'end stability, 5' end stability, secondary structures why is the formation of secondary structures in primers especially undesirable - reduces primer available to use to make product standard denaturation temp ...

PCR allows for: - amplification of minute amounts of template DNA can generate thousands or millions of copies of that sequence What is one of the most important parameters for a successful PCR? - well designed primers Name 3 variables that influence the success of a PCR reaction - 1. quality...