ACS BIOCHEMISTRY EXAM Questions and Answers

State the central dogma of genetics Ans- DNA can self replicate via translation DNA is coded into RNA via transcription RNA is made into proteins via translation Structure of a nucleotide Ans- 5 carbon sugar, phosphate group, and a nitrogenous base Name the monomer of nucleic acids Ans- nucle...

Henderson-Hasselbach Equation Ans- pH = pKa + log ([A-] / [HA]) Salting Out (Purification) Ans- Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography Ans- Separates sample based on si...

Henderson-Hasselbach Equation Ans- pH = pKa + log ([A-] / [HA]) Salting Out (Purification) Ans- Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography Ans- Separates sample based on si...

Henderson-Hasselbach Equation Ans- pH = pKa + log ([A-] / [HA]) Salting Out (Purification) Ans- Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography Ans- Separates sample based on si...

Henderson-Hasselbach Equation Ans- pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis Ans- Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) Ans- Changes soluble protein to solid precipitat...

nucleotides Ans- monomeric unit for nucleic acids and are composed of nitrogenous base, 5-carbon sugar and a phosphate nucleic acids are biopolymers of Ans- nucleotide monomers DNA Ans- -more stable - carrier of genetic information - double stranded RNA Ans- - less stable - intermediate ...

Helicase Ans- Catalyzes ATP-dependent unwinding of DNA double helices What is the helicase in E. Coli? Ans- DNAB Single-Stranded Binding Protein Ans- Binds to unwound strands of DNA to prevent reannealing DNA Polymerase Ans- Enzyme responsible for DNA replication Uses ssDNA as a template and...

Which Amino Acids will be charged at pH 7? Ans- DEHRK: Aspartic Acid (Asp), Glutamic Acid (Glu), Histidine (His), Arginine (Arg), Lysine (Lys) Which amino acids will have a negative charge at pH 7? Ans- D&E: Aspartic Acid (Asp) and Glutamic acid (Glu) Which amino acids will have a positive cha...

Glycolysis Ans- Near-universal pathway in which glucose molecule is oxidized to two molecules of pyruvate, with energy conserved as ATP and NADH Glycolytic breakdown Ans- Glucose is the sole source of metabolic energy in some mammalian tissues and cell types Fermentation Ans- general form f...