Portage Learning BIO 171 lab 8 lab exam .

Lab 8 Quiz – Microbiology Question 1 The word ‘antigen’ is actually a combination for what two words? Your Answer: Antibody pathogen Antibody-generating Question 2 For the ELISA assay depicted below, which step (A-E) contains only specifically bound antigens (aka a 'pure' population)? Your Answer: In step C you see only specifically bound antigens, aka a pure population. In step B you see specific and non-specific antigens, but then the wash step removes the non-specific or unbound antigens, leaving only the specific antigen which is present in step C. Specifically bound antigens are present in step C. Specific and non-specific antigens are present in B but then the wash step removes the non-specific (unbound) antigens, leaving only the specific population seen in step C. Question 3 What would happen in terms of the colorimetric readout if you forgot to do the wash step after Step D (above)? Your Answer: If you forgot to do the was step after Step D, all the wells would have a dark color. This is because the intensity of the readout is dependent upon the amount of the labeled secondary antibody present in the well. Since the unbound secondary antibody was not washed away, all the wells would react the same. All of the wells would have a dark color. The intensity of the readout is dependent upon the amount of the labeled secondary antibody present in the well. Since the unbound secondary antibody was not washed away, all wells would react equally. Question 4 In a western blot ____________ are separated based on its size. Your Answer: Proteins proteins Question 5 When setting up a western blot, what is the purpose of the blocking step post-transfer? This study source was downloaded by from CourseH on :37:56 GMT -06:00