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BIOS 242 Week 7 iLab: EPIDEMIOLOGY
EPIDEMIOLOGY
Introduction
I conducted a series of biochemical tests to identify the bacterium responsible for the
situation presented in the case study. This week’s case study illustrates a situation that an
epidemiologist might need to solve. A rare expensive monitor lizard dies and the
following day a Gila monster and rare Tibetan Newt died as well. A bacteriological
survey of the reptiles and amphibians was obtained with the end result being salmonella
bongori with gram negative rods.
Procedure
You will conduct four biochemical tests (Acid from Glucose, Gas from Glucose, Lysine
decarboxylase, Malonate Utilization, Salicin fermentation, Sorbital fermentation, Growth
in KCN, and Dulcitol fermentation) on your unknown bacterium sample. You may have
noted that you have conducted several of these tests in previous weeks.
Conduct Acid from Glucose and Gas from Glucose Tests:
1. Click the New Unknown button and type Epidemiology in the “Enter a Label” field.
Select Epidemiology 1 from the Subgroup dropdown menu.
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2. Click the box allowing autoinoculation. Click OK. iLab: Epidemiology Page 3
3. Record your case study.
4. Record the results of the Gram Stain.
5. Open the Acid from Glucose Test from the Biochemical Tests Reference Book.
Review the steps of this test and determination of test results. NOTE: You have
conducted both of these tests in a previous week’s experiment.
6. Select Phenol Red Glucose Broth Durham Tube as your medium. Label the medium:
PhenolRedGlucose. Click OK.
7. Inoculate your medium. Place in 37 degree incubator. Click Next Day.
8. Retrieve sample from incubator. Observe and record the results for Acid from Glucose
and Gas from Glucose tests.
Conduct Lysine Decarboxylase Test:
1. Open the Lysine decarboxylase Test from the Biochemical Tests Reference Book.
Review the steps of this test, including that you will record results after 24 and 48 hours.
Make note of how you will determine the results of this test.
2. Select Lysine decarboxylase Broth as your medium. Label the medium: LysineD.
Click OK.
3. Inoculate your medium. Place in 37 degree incubator. Click Next Day.
4. Retrieve sample from incubator. Note color of medium. Return your inoculated sample
to the 37 degree incubator. Click Next Day.
BIOS 242 Week 7 iLab: EPIDEMIOLOGY
EPIDEMIOLOGY
Introduction
I conducted a series of biochemical tests to identify the bacterium responsible for the
situation presented in the case study. This week’s case study illustrates a situation that an
epidemiologist might need to solve. A rare expensive monitor lizard dies and the
following day a Gila monster and rare Tibetan Newt died as well. A bacteriological
survey of the reptiles and amphibians was obtained with the end result being salmonella
bongori with gram negative rods.
Procedure
You will conduct four biochemical tests (Acid from Glucose, Gas from Glucose, Lysine
decarboxylase, Malonate Utilization, Salicin fermentation, Sorbital fermentation, Growth
in KCN, and Dulcitol fermentation) on your unknown bacterium sample. You may have
noted that you have conducted several of these tests in previous weeks.
Conduct Acid from Glucose and Gas from Glucose Tests:
1. Click the New Unknown button and type Epidemiology in the “Enter a Label” field.
Select Epidemiology 1 from the Subgroup dropdown menu.
, 2
2. Click the box allowing autoinoculation. Click OK. iLab: Epidemiology Page 3
3. Record your case study.
4. Record the results of the Gram Stain.
5. Open the Acid from Glucose Test from the Biochemical Tests Reference Book.
Review the steps of this test and determination of test results. NOTE: You have
conducted both of these tests in a previous week’s experiment.
6. Select Phenol Red Glucose Broth Durham Tube as your medium. Label the medium:
PhenolRedGlucose. Click OK.
7. Inoculate your medium. Place in 37 degree incubator. Click Next Day.
8. Retrieve sample from incubator. Observe and record the results for Acid from Glucose
and Gas from Glucose tests.
Conduct Lysine Decarboxylase Test:
1. Open the Lysine decarboxylase Test from the Biochemical Tests Reference Book.
Review the steps of this test, including that you will record results after 24 and 48 hours.
Make note of how you will determine the results of this test.
2. Select Lysine decarboxylase Broth as your medium. Label the medium: LysineD.
Click OK.
3. Inoculate your medium. Place in 37 degree incubator. Click Next Day.
4. Retrieve sample from incubator. Note color of medium. Return your inoculated sample
to the 37 degree incubator. Click Next Day.
