Other
BIOS 242 Week 6 iLab: Specific Immunity
- Course
- Institution
BIOS 242 Week 6 iLab: Specific Immunity
[Show more]
Seller
Follow
PerfectStudy
Reviews received
Content preview
1BIOS 242 Week 6 iLab: Specific Immunity
SPECIFIC IMMUNITY
Introduction
This lab experiment was done to see if there were any pathogens in the watermelon that a
student ate after it falling in the barn. My case study involved students being at a barn and
everyone enjoying and playing games. One of the students dropped his slice of
watermelon in the barn. He picked it up, and simply just rinsed it off and proceeded to eat
it. The sponsor knew the watermelon would be harmful, so took the slice and put it in a
plastic bag and taken to the lab. The results of that stain were that it had gram negative
rods.
Procedure
You will conduct four biochemical tests (Acid from Glucose, Gas from Glucose, Lactose
Fermentation, Indole Production, Lysine decarboxylase, and Malonate Utilization) on
your unknown bacterium sample. You may have noted that you have conducted several
of these tests in previous weeks.
Conduct Acid from Glucose and Gas from Glucose Tests:
1. Click the New Unknown button and type SpecificImmunity in the “Enter a Label”
field. Select Specific Immunity from the Subgroup dropdown menu.
2. Click the box allowing autoinoculation. Click OK.
3. Record your case study. iLab: Specific Immunity Page 3
, 2
4. Record the results of the Gram Stain.
5. Open the Acid from Glucose Test from the Biochemical Tests Reference Book.
Review the steps of this test and determination of test results. NOTE: You have
conducted both of these tests in a previous week’s experiment.
6. Select Phenol Red Glucose Broth Durham Tube as your medium. Label the medium:
PhenolRedGlucose. Click OK.
7. Inoculate your medium. Place in 37 degree incubator. Click Next Day.
8. Retrieve sample from incubator. Observe and record the results for Acid from Glucose
and Gas from Glucose tests.
Conduct Lactose Fermentation Test:
1. Open the Lactose Fermentation Test from the Biochemical Tests Reference book.
Review the steps of the test and determination of test results. NOTE: You have conducted
both of these tests in a previous week’s experiment.
2. From the Media dropdown menu, select Phenol Red Lactose Broth.
3. Enter PhenolRedLactose in the Medium Label window to label your sample. Click
OK.
4. Inoculate your medium. Place in 37 degree incubator. Click Next Day.
5. Retrieve sample from incubator. Observe and record results.
Conduct Lactose Fermentation Test:
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller PerfectStudy. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $10.49. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
53340 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now