BIOS 251 Week 4 Lab-Microscopy Learning Objectives: Understand different microscopy techniques and their limitations Identify various cell types and cellular structures Understand staining techniques Understand coeliac disease and intestinal inflammation Introduction: Histology is an important tool...
understand different microscopy techniques and their limitations identify various cell types and cellular structures
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Chamberlain College Of Nursing
BIOS 251 (BIOS251)
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BIOS251 OL, Week 4 Lab
Name: Veronica Rongo
OL Lab 4: Microscopy
Learning Objectives:
Understand different microscopy techniques and their limitations
Identify various cell types and cellular structures
Understand staining techniques
Understand coeliac disease and intestinal inflammation
Introduction:
Histology is an important tool of biology which helps us understand how tissues are
structured and how they function. This is helpful when we are investigating diseased or
malformed tissues.
In this simulation you will examine a chicken intestine sample that is infected with a
retrovirus and observe how the virus infects the lymphocytes (the cells that provide
immune protection) and how it inhibits inflammation. Inflammation is a sign of tissue
damage. You will use different microscopic techniques to examine chicken intestine
slide.
Try out the electron microscope
Electron microscopes can be used to visualize objects that are too small to see when
using a light microscope—for example the microvilli. You can zoom in and out to
observe different cellular structures such as mitochondria and the cell. Using the 5X
magnification, you will identify the villus, and then proceed with higher magnifications to
identify smooth muscle, extracellular tissue, epithelial cells, Goblet cells and the nuclei.
Learn about fluorescence staining techniques
For example, by staining the DNA with DAPI, you can easily identify a cell’s nucleus. In
this part of the lab, you will examine a chicken intestine sample that is infected with a
retrovirus and observe how the virus infects the lymphocytes and how it inhibits
inflammation. The retrovirus can be further developed as medicine for coeliac disease.
Part 1: Complete Labster Microscopy
Part 2: Report and Reflection
Purpose: Describe in your own words and in complete sentences, the purpose of this
experiment.
We examined a chicken intestine that was infected with a retrovirus and observe how
the virus infects the lymphocytes and how it inhibits inflammation. We used an electron
microscope to visualize objects that are too small to see when using a light microscope.
We went over the different things that are in a cell and their function while locating them
with the electron and light microscope.
Observations: List 3 observations you have made in this simulation.
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