Class notes
Must Know in Dealcoholization and Clearing - Histopathology
- Course
- Institution
The document contains Must-know information about dealcoholization and clearing for board exam review.
[Show more]
Seller
Follow
iskodyante
Content preview
DEALCOHOLOZATION / CLEARING▪ Reagents makes tissue TRANSPARENT, hence the name CLEARING, however, not all reagents can make
tissue transparent.
▪ Reagents will also improve the tissue’s REFRACTIVE INDEX
▪ Incomplete clearing = incomplete H and E staining and poor nuclear chromatin patterns
▪ Prolong clearing time = causes tissue to become brittle
▪ Removal of dehydrating agent from tissues to replaced it with a medium that will dissolve the wax with
which the tissues is to be impregnated.
CLEARING AGENTS
1. XYLENE
▪ ROUTINE CLEARING AGENT because it is fast-acting (often used)
▪ With rapid clearing time 30 mins – hours
▪ NOT suited for nervous tissues & lymph nodes
▪ It becomes milky when an incompletely dehydrated tissue is immersed in it
2. BENZENE
▪ Also, fast-acting that’s why it is suited for urgent biopsies
▪ Highly flammable and carcinogenic
▪ Prolong exposure can damage the bone marrow leading to aplastic anemia
3. TOLUENE
▪ Substitute for Xylene and Benzene but not as fast as those 1-2 hours
▪ Disadvantage: Acidifies in a partially filled vessel (nababago yung pH nito if the container is not
fully filled)
4. CHLOROFORM: Used for dense tissues like the uterus
▪ NOT A ROUTINE CLEARING AGENT but it is recommended for clearing tough tissues (skin, fibroid
and decalcified tissues) and can also be used for nervous tissues, lymph nodes and embryos
▪ Tissues tend to float, remedy Wrap tissue in a gauze to keep it at the bottom
▪ Toxic to LIVER
▪ Does not make tissue transparent; Tissues placed on this do not become translucent
▪ Can lead to wax deterioration
5. CEDARWOOD OIL: Also used for dense tissues like the uterus
▪ Clearing time: 2-3 days
▪ Extremely slow clearing agent
▪ Recommended for cytological studies like smooth muscles of skin and CNS tissues
6. ANILINE OIL
▪ Recommended for clearing embryos, insects, and other very delicate spx
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller iskodyante. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $5.49. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
83637 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now