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Biochemistry experiment
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Experiment WorksheetQualitative Analysis of Fats and Oils
A. Write down the result and your observation based on the test that performed in the
link provided.
Solubility Test (water)
Samples Results Observation
Dhesi ghee It floated only. It is After the addition of 5 ml of
immiscible (not mixable) in water and upon shaking the
water. It means oil or fat is solution, the sample is
present. immiscible.
Vegetable ghee It floated only. It is The sample is immiscible
immiscible (not mixable) in after adding 5 ml of water
water. It means oil or fat is and shaking the solution.
present.
Refined oil It floated only. It is The sample is immiscible
immiscible (not mixable) in when mixed with 5 ml of
water. It means oil or fat is water and shaken.
present.
Solubility Test (alcohol)
Samples Results Observation
Dhesi ghee It formed a lower layer. After adding 5 ml of
After heating, It was alcohol, the solution is
dissolved. partly miscible that forms a
lower layer which dissolves
upon heating.
Vegetable ghee It formed a lower layer. After adding 5 ml of
After heating, It was alcohol, the solution
dissolved. becomes somewhat
, miscible, forming a bottom
layer that dissolves when
heated.
Refined oil It formed a lower layer. When mixed with 5 ml of
After heating, It was alcohol, it creates a
dissolved. somewhat miscible layer
that dissolves when heated.
Solubility Test (chloroform)
Samples Results Observation
Dhesi ghee It is miscible (mixable) in The sample is diluted with
chloroform. solvent after being mixed
with 5 ml of chloroform and
shaken.
Vegetable ghee It is miscible (mixable) in After shaking the sample
chloroform. with 5 ml of chloroform, it
is diluted with solvent.
Refined oil It is miscible (mixable) in When 5 ml of chloroform is
chloroform. added and shaken, the
sample is combined with
the solvent.
Translucent Spot Test
Samples Results Observation
Dhesi ghee A translucent spot appeared A translucent spot can be
on the filter paper. seen on the filter papers.
When the filter paper is
placed in the front of the
lightning candle and moved
over the flame, the light will
be partly visible only
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