BIO 2923 TEST 3 (Bacteria, Growth medium, d., E.)/ with answer key/ 100% CORRECT SOLUTIONS
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Course
BIO 2923
Institution
Northwest Mississippi Community College
BIO 2923 TEST 3 BIO 2923 TEST 3 Student: 1. The Five I's of studying microorganisms include all of the following except: A. inoculation B. incubation C. infection D. isolation E. identific ation 2. All of the following are examples of different types of microbiological media except: A. broth B. enr...
1. The Five I's of studying microorganisms include all of the following except:
A. inoculation
B. incubation
C. infection
D. isolation
E. identification
2. All of the following are examples of different types of microbiological media except:
A. broth
B. enriched
C. agar
D. petri dish
E. gel
3. The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium
is:
A. isolation
B. inoculation
C. immunization
D. infection
E. contamination
4. Which of the following is essential for development of discrete, isolated colonies?
A. broth medium
B. differential medium
C. selective medium
D. solid medium
E. assay medium
5. A pure culture contains only:
A. one species of microorganism
B. bacteria
C. a variety of microbes from one source
D. a variety of species from the same genus
E. none of the choices are correct
6. Which of the following will result when 1% to 5% agar is added to nutrient broth, boiled and cooled?
A. a pure culture
B. a mixed culture
C. a solid medium
D. a liquid medium
E. a contaminated medium
7. Agar is an important component of media because
A. bacteria require agar to grow
B. agar inhibits mold growth
C. agar provides a solid surface for bacterial growth
D. agar prevents contamination
E. all of the choices are correct
,8. A microbiologist inoculates Staphylococcus aureus into a culture medium. Following
incubation, both Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in
this culture. What is the most likely explanation?
A. the microbiologist used too much inoculum
B. the culture is contaminated
C. the incubation temperature was incorrect
D. the culture medium must be selective
E. the culture medium must be differential
9. Bacteroides is inoculated into a pour plate. After incubation, colonies are only seen within the agar and
not on the surface. Bacteroides must be:
A. aerobic
B. axenic
C. anaerobic
D. in selective media
E. in differential media
10. A microbiologist inoculates Staphylococcus epidermidis and Escherichia coli into a culture medium.
Following incubation, only the E. coli grows in the culture. What is the most likely explanation?
A. the microbiologist used too much inoculum
B. the culture is contaminated
C. the incubation temperature was incorrect
D. the culture medium must be selective
E. the culture medium must be differential
11. Which method often results in colonies developing down throughout the agar and some colonies on the
surface:
A. streak plate
B. spread plate
C. pour plate
D. all of the choices are correct
E. none of the choices are correct
12. A common medium used for growing fastidious bacteria is:
A. blood agar
B. trypticase soy agar
C. mannitol salt agar
D. MacConkey medium
E. a reducing medium
13. A nutrient medium that has all of its chemical components identified and their precise concentrations
known and reproducible, would be termed:
A. complex
B. reducing
C. enriched
D. synthetic
E. none of the choices are correct
14. A reducing medium contains:
A. sugars that can be fermented
B. extra oxygen
C. hemoglobin, vitamins or other growth factors
D. substances that remove oxygen
E. inhibiting agents
, 15. Which type of medium is able to distinguish different species or types of microorganisms based on an
observable change in the colonies or in the medium?
A. differential
B. selective
C. enumeration
D. enriched
E. reducing
16. A microbiologist decides to use a nutrient medium that contains thioglycollic acid. What type of microbe
is she attempting to culture?
A. fastidious
B. gram positive
C. anaerobe
D. gram negative
E. aerobic
17. Differential media results in which of the following growth characteristics?
A. different color colonies
B. different media color post incubation
C. precipitates
D. gas bubbles
E. all of the choices are correct
18. A reducing media is used to culture
A. fastidious organisms
B. aerobic organisms
C. anaerobic organisms
D. any pathogenic organisms
E. none of the choices are correct
19. For which bacterial genus is mannitol salt agar selective?
A. Salmonella
B. Streptococcus
C. Neisseria
D. Staphylococcus
E. Escherichia
20. A microbiologist must culture a patient's feces for intestinal pathogens. Which of the following would
likely be present in selective media for analyzing this fecal specimen?
A. NaCl
B. sheep red blood cells
C. bile salts
D. thioglycollic acid
E. peptone
21. Which of the following characteristics refers to the microscope's ability to show two separate entities as
separate and distinct?
A. resolving power
B. magnification
C. refraction
D. all of the choices are correct
E. none of the choices are correct
22. Which of the following magnifies the specimen to produce the real image of the specimen?
A. condenser
B. objective lens
C. ocular lens
D. body
E. nosepiece
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