100% satisfaction guarantee Immediately available after payment Both online and in PDF No strings attached
logo-home
Biochemistry Transcription Concept Map $7.49   Add to cart

Case

Biochemistry Transcription Concept Map

 6 views  0 purchase
  • Course
  • Institution
  • Book

Case study book Harper's Illustrated Biochemistry Thirty-First Edition of Victor Rodwell, David Bender - ISBN: 9781259837944, Edition: 31, Year of publication: - (concept map)

Preview 1 out of 3  pages

  • August 27, 2022
  • 3
  • 2021/2022
  • Case
  • Dr.
  • A
avatar-seller
DNA: RNA is synthesized
Template strand – read at 3’ to 5’ from 5’ to 3’ end
Coding strand – same sequence as
RNA produces with U in place of T


Biosynthesis of
Requires ATP, GTP, RNA from DNA
CTP, UTP and Mg2+ DNA in form of chromosomes
(more compact) & presence of
histones --- more complicated
TRANSCRIPTION


PROKARYOTES
Catalyzes RNA EUKARYOTES
synthesis

Pol I: synthesizes major
RNA Pol: synthesizes mRNA, rRNA, rRNA precursors
tRNA
Core enzyme – α2ωββ’
Holoenzyme – α2ωββ’σ RNA Polymerase Pol II: synthesizes
σ subunit recognizes promoter mRNA precursors


Most extensively studied
α – assembles enzyme, recognizes
GTFs
ω – restores denatured RNA pol First base Region of Pol III: synthesizes
ββ’ – combine to form active site
incorporated to template strand tRNA, 5S rRNA, small
σ – recognizes promoter
RNA chain: +1 to which RNA precursors
position, TSS polymerase
binds

Pribnow box: -10 region
Core promoter: -35 element TATA box: -25; TATAAAA
or region Promoter GAAT box: -80; GGCCAATCT
Extended promoter: UP GC box: variable; GGGCGG
element (-40 to -60)


Consensus
Upstream: - sequence - shows Promoter – rich in AT
Downstream: + the nucleotides with 2 H-bonds only
σ recognizes promoter; bridging most commonly
core promoter to RNA pol; found at each
closed complex is formed Most studied position
and Formation of preinitiation
controlled
complex, which normally
σ initiates separation of DNA contains Pol II and general TFs
strands; open complex is
formed
TFIID recognizes TATA box;
Initiation TFIIA binds and interacts with
DNA and TFIID; TFIIB binds
bridging BP and Pol II; TIIF
β and β' bind to ssDNA; ATP or binds to Pol II to suppress
Transcription
GTP (first RNA base) binds to nonspecific binding; TFIIE
factor – regulating
TSS (+1 position) interacts with
protein but not a unphosphorylated Pol II; TFIIH
part of RNA pol exhibits helicase activity

The benefits of buying summaries with Stuvia:

Guaranteed quality through customer reviews

Guaranteed quality through customer reviews

Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.

Quick and easy check-out

Quick and easy check-out

You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.

Focus on what matters

Focus on what matters

Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!

Frequently asked questions

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

Satisfaction guarantee: how does it work?

Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.

Who am I buying these notes from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller compiler. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy these notes for $7.49. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

72042 documents were sold in the last 30 days

Founded in 2010, the go-to place to buy study notes for 14 years now

Start selling
$7.49
  • (0)
  Add to cart