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Samenvatting Theorie voor de praktijk Microbiologie leerjaar 2 $6.32   Add to cart

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Samenvatting Theorie voor de praktijk Microbiologie leerjaar 2

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De theorie voor de praktijk microbiologie samenvatting gaat over de achtergrondinformatie van de technieken die je in de praktijk gaat uitvoeren en die ook worden getoetst op het tentamen. Hierbij gaat het om de technieken en platen die in de lessen worden gebruikt. Zo wordt de samenstelling van ee...

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  • September 19, 2022
  • 14
  • 2021/2022
  • Summary

1  review

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By: bicerelirem18 • 5 months ago

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Microbiologie theorie voor de praktijk les 1

-Praktijk microbiologie:
>




-Microscopische morfologie:
>Gram positieve en gram negatieve bacteriën:
*Coccen, staven en spiraalvormige
*Groepering speelt een rol bij de coccen
~Duplo, of streng, of groepjes
van vier of kubisch of heel
random groepering (druiventrosjes)
*




-Macroscopische morfologie:
>



*Coccen vormen kleine kolonies en staven vormen
grote kolonies




-Media:
>Selectieve / differentiële media:
*Bloedagar


Made by: Iris Gülcher

, *MacConkey
*DCLS-agar
*XLD-agar
*CCDA
-Diagnose van urineweginfecties:
>Dag 1:
*Gramkleuring (microscopische morfologie)
*Urinekweek (macroscopische morfologie)
~Bloedagar
~MacConkey-agar
>Dag 2-3:
*Bepaling/Identificatie en antibioticaresistentie
-Bloedagar:
>Erg verrijkt (veel voedingsstoffen)
>Pepton/digesten/extracten/hydrolysaten
>




>Electief/differentieel
>Losse koloniës
>-hemolyse (greening):
*Oxidatie hemoglobine -> methemoglobine
>β-hemolyse (complete lysis):
*Hemolysines (cytotoxine)
*Emolyse: afbraak van erytrocyten
>γ-hemolyse (indifferent)
-MacConkey agar (selectief en electief/differenieel (lactose fermentatie)):
>Selectief met gramnegatieve bacteriën (positieve bacteriën kunnen er niet op groeien)
*Met uitzondering van (staan in protocollen bundel) (pinpoint groei)
*Zijn vaak grampositieve die terug worden gevonden in de urinekweek
>E.coli kan lactose fermenteren
>




>pH 7.4 ± 0.2 bile salts (“galzouten”) = natriumdeoxycholaat
>




Made by: Iris Gülcher

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