Unit 2- Practical Scientific Procedures and Techniques
Summary
Summary BTEC Applied Science: Unit 2, Learning Aim C
12 views 0 purchase
Course
Unit 2- Practical Scientific Procedures and Techniques
Institution
PEARSON (PEARSON)
Practical scientific procedures and technique learning aim B (calorimetry). this assignment achieved a grade distinction
NOTE: THIS ASSIGNMENT SHOULD ONLY BE USED AS A GUIDE. IS UPLOADED TO SHOW WHAT DISTINCTION LEVEL SHOULD LOOK LIKE NOT TO BE COPIED. IN CASE OF PLAGARISM MAY LEAD TO REVOKED OF ...
Unit 2- Practical Scientific Procedures and Techniques
All documents for this subject (365)
Seller
Follow
himashi123
Content preview
Introduction
Chromatography is the separation technique which is commonly used to separate or identify
unknown components, or solutes, of a mixture all depending on the idea of the relative amount
of solute distributed between a mobile phase, and a continuous stationary phase. In almost all
techniques of chromatography the stationary phase is either a solid or a liquid. (Giddings, 2020)
The distinct pigments/substances are identified through a calculation which is known as rf
value. To find the rf value of a substance you find the distance travelled by the components and
divide it by the distance travelled by the solvent (Fink, Cline and Fink, 1963)
Distance travelled by pigment
RF VALUE=__________________________________
Distance travelled by the solvent
Different types of chromatography techniques are:
- Paper chromatography
- Column chromatography
- Ion-exchange chromatography
- Thin Layer chromatography
- Gas chromatography
Coskun, 2016)
Along with these there are several different types of chromatography techniques that are used
in different industries and for a variety of reasons which cannot be conducted for example a
school might more likely conduct a paper chromatography due to the range of complexity
compared to a forensic scientist may ustilise gas chromatography. Throughout this report, 3
experiments will be carried out: Paper chromatography of spinach leaves, thin layer
chromatography of spinach leaves and paper chromatography of amino acids. Overall, these
experiments will help us find the most efficient and judge the accuracy of the rf values
compared to research statistics
, Report 1: Paper Chromatography of Spinach
Introduction:
Paper chromatography is a partition principle which is mainly used to separate a variety of
substances or identify unknown substances in analytical chemistry. This is where the filter
paper also known as ‘chromatography’ paper is the stationary phase and where the liquid is the
mobile phase (Clegg, 1950). Paper chromatography relies on the idea of the solvent going
through substances to figure out if the substance being tested is pure or impure.
The experiment carried out solvent, propanone will be the mobile phase and the
chromatography paper will be the stationary phase.
Aim
The whole aim of this experiment was to investigate the different pigments that form green
found in green plants for which in this case was spinach leaves.
, Risk Assessment
Equipment Risk Safety Risk Level
Propanone Flammable; Must Keep away from HIGH
(Solvent) be kept away yourself and away
from naked from electrical
flames. Causes appliances. Wear
irritation to eyes eye safety goggles
and skin
Glassware Glassware might Wear eye safety MEDIUM
break and cut goggles. Keep all
someone glassware away
from table edges
so it doesn’t know
over.
Mortar and pestle Heavy and fragile. Keep away from LOW
edges of table and
handle with care
Equipment List
- 100cm3 beaker
- Mortar and pestle
- Teat pipette
- Chromatography paper
- Propanone
- Sand
- Cut up leaves (Spinach)
- Capillary tube
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller himashi123. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $9.78. You're not tied to anything after your purchase.
