MLT Urinalysis questions with complete solutions

pH Reaction Correct Answer: Double indicator Methyl Red and Bromethymol Blue Protein Reaction Correct Answer: Protein error of indicator Glucose Reaction Correct Answer: Glucose Oxidase Ketones Reaction Correct Answer: sodium nitroprusside Blood Reaction Correct Answer: peroxidase activity of Heme Myoglobin Reaction Correct Answer: Ammonium sulfate screen centrifuge sample and then test with Blood reagent strip positive = Myoglobin Bilirubin Reaction Correct Answer: Diazo reaction Diazonium salt azobilirubin Urobilinogen Reaction Correct Answer: Para-dimethyl Elrich's Nitrite Reaction Correct Answer: Greiss Reaction Diazotization of nitrite w/ aromatic amino Diazonium salt Leuocyte Reaction Correct Answer: Ester and Diazonium salt Ester + aromatic compound Diazonium salt pH erroneous results Correct Answer: improper storage (increase) contaminated container Improper technique leaching buffer Protein false positives Correct Answer: Highly buffered alkaline urine Allowing pad to stay in contact with urine too long may leach out buffer Contamination of container with ammonium compounds or detergents alkaline cleaning agents Highly colored substances, eg pyridium, beets (mask color) Protein false negatives Correct Answer: Globulin Myoglobin Bence Jones protein Highly colored substances, eg pyridium, beets (mask color)