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Anatomy and Physiology 101 Lab 2 Microscope, Cellular Structure, The Cell Cycle Labs 4, 5, and 7 in manual Textbook Figures 3.3, 3.7, and 3.37 Table 3.3 and 3.5

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Anatomy and Physiology 101 Lab 2 Microscope, Cellular Structure, The Cell Cycle Labs 4, 5, and 7 in manual Textbook Figures 3.3, 3.7, and 3.37 Table 3.3 and 3.5 Lab Exercise 4 - Care and Use of the Compound Microscope The microscope is an essential tool for the study of structures too small to see with the naked eye. Objectives- In this lab you will learn the parts of a microscope, the function of each part, how to calculate total magnification, and how to place a slide in position and focus on an image. Directions- For this exercise follow the directions in the manual. You are responsible for the information in the manual from 1-9 under Procedure. However you will not be doing any measurements with a ruler or use of the thread slide. You will be using prepared slides of squamous cells. You are not responsible for the information on wet mount slides (Procedure B). To learn the parts of the microscope use the diagram for reference and the handout provided. LAB REPORT 4 – PART A & D Lab Exercise 5 - Cell Structure and Function The 3 major parts of a cell are the cell membrane that encloses the cell, the cytoplasm and the organelles contained within, and the nucleus. Objectives- In this exercise you will learn the parts of the cell and the functions of each part. You are responsible for this on both diagrams and the cell models. Directions- Locate the following structures on the cell model. Functions of each are also given. Description and Function 1. Cellmembrane Doublelayerofphospholipidmoleculesmakingasemi- permeablemembrane,alsocontainscholesterolwhich maintainsstructureofthemembraneandproteinswhich helpsregulate what enters and leaves cell 2. Organelles in cytoplasm Mitochondria Cell powerhouse- small cigar-shaped structures that produce energy for the cell Endoplasmic reticulum (ER) A series of flattened membranous sacs that extend from the nucleus to the cell membrane. There are 2 types of ER. 1. Rough (RER) Contains on surface ribosomes which make proteins. 2. Smooth (SER) Contains enzymes needed for lipid synthesis Golgi apparatus Flattened sacs smaller than ER, packages and ships primarily proteins out of cell, may also combine proteins with carbohydrates to make glycoproteins. The UPS of the cell. Centrosome Consists of 2 centrioles, aid in cell reproduction by making spindle fibers Ribosomes Small particles composed of RNA and proteins, may be free or attached to RER, produce proteins Lysosomes Membranous sacs, cell garbage disposal Vesicles Membranous sacs, contain substances that just entered cell or substances prepared to leave cell Peroxisomes Membranous sacs, breaks down organic molecules 3. Nucleus Control center of cell Nuclear envelope Double layer membrane that contains pores, regulates what enters and leaves the nucleus Nucleolus Nucleus may contain 1 or more, form ribosomes Chromatin Long, thin strands of protein and DNA, contains the blueprint for cell activity which is primarily synthesizing proteins The following organelles are found in cells but are not present on our model but you do need to know about them and identify them on the diagram. Cilia Projections off edge of cell membrane, move substances along surface of cell Flagella Long, single projection off cell membrane, moves entire cell Microfilaments and microtubules Thin protein strands within cytoplasm, help maintain structure of cell and move substances throughout cell

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