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Collin College BIOL 1408 Unit 8 Biology Lab Report Enzymes
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- BIOL 1408
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- Collin College
Collin College BIOL 1408 Unit 8 Biology Lab Report Enzymes
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Running head: Unit 8 Enzymes 1Unit 8 Biology Lab Report: Enzymes
Collin College Biology 1406
Author Note
Laboratory Report on Biology Unit 8 Enzymes and the Effects of Lactose Intolerance
, Unit 8 Enzymes 2
Abstract
The experiments and procedures outlined in this report discuss the effects of lactose
intolerance which affects millions of people worldwide. The disaccharide Lactose is made
of up the monomers beta-D-galactose and beta-D-glucose. It is an enzyme that is found in
various organs throughout the bodies of mammals, and is used as an energy source in cells.
There have been many advances in the in the production of enzymes that help individuals
who are affected by lactose intolerance. These products assist people who lack these
enzymes in breaking down Lactose. The purpose of this laboratory experiment is to test
Lactose for the chemical properties of the enzyme, and to arrive at a conclusion as to the
determination of if the enzyme Lactase came from human or bacterial cells. The first
experiment involves the testing of the effects of temperature on enzyme activity, by
exposing the enzyme to different set levels of temperature and recording the effect. It is
hypothesized that the enzyme will follow denaturation, in that it will be effective to a
certain extent until the temperature becomes too high and begins to denature. The second
experiment encompasses the effects of pH on enzyme activity to discover the effects that
different pH levels have on enzyme activity. It is hypothesized that the effects will be
similar to the effects of denaturization as well, working effectively to an extent to when the
pH is either too high or too low will begin to denature. The third experiment will specify if
the enzyme lactase will be most likely to interact or bind with lactose compared to maltose.
The final experiment will determine what the specific cofactors of the enzyme lactase are,
as well as the role that metal ions will have on the activity of lactase.
Keywords: Lactose, Lactase, enzyme, cells, hypothesize.
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