BSC2010 LAB 8 questions with complete top solutions

BSC2010 LAB 8 questions with complete top solutions What word fits in the gap? When a gene is expressed... Is produced. MRNA All genes are expressed in cells. Is this statement true? No only genes that are relevant for the particular cell are expressed If mRNA a from gene A is very abundant in the fat tissues from the obese pig and rare in the fat tissues of the lean pig it shows as that Gene A is more expressed in obese animals Why do we keep tissue samples for rna work in liquid nitrogen To preserve the RNA which quickly degrades at room temperature That RNA content in a cell Includes both coding mRNA and several types of non-coding rna Before we start, do you know Where is most of the MRNE located In the cytoplasm The lysis buffer use for RNA extraction contains Guanidinium isothiocyanate and phenol What does the lysate consist of Rna, dna, proteins, and cellular debris What do you measure at 230, 260 and 280 nm respectively Chemicals, rna/dna, protiens At 260/280 should be between 1.8 and 2.1. What does it mean if we have a ratio that his lower than 1.8 They are a lot of proteins in the rna sample What will a spectrophotometer measure Purity and quantity What you see in the gel if the RNA is intact two bands corresponding to rna Why did we went to specifically extract that MRNA only Because mRNA represent the genes that are expressed in I'll be translated into proteins What is the common sequence that all mRNA share Poly A