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Samenvatting medische immunologie H4 en H7 $3.23   Add to cart

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Samenvatting medische immunologie H4 en H7

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Deze samenvatting van het vak medische immunologie bevat een overzicht van hoofdstuk 4 over de laboratoriumtechnieken in een immunologisch labo en een samenvatting met eigen figuren van H7 over de ontwikkeling en de activatie van lymfocyten.

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  • June 16, 2023
  • 5
  • 2022/2023
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H4: Immunologische
laboratoriumtechnieken
1. Agglutinatietechnieken
a. Hemagglutinatie
b. Latexagglutinatie
c. Bacteriële agglutinatie

2. Precipitatietechnieken
a. Vloeibare fase systemen
i. Turbidimetrie
ii. Nefelometrie
b. Vaste fase systemen
i. Radiale immunodiffusie
ii. Immuno-elektroforese

3. Biologische activiteit
a. Complement fixatie test
b. Virus neutralisatie test
c. Hemagglutinatie inhibitie test

4. Gemerkte antilichamen (vloeibaar)
a. Enzyme
i. Capture ELISA
ii. Competitieve ELISA
b. Radioactief isotoop
i. Competitieve RIA (RIST, PRIST, RAST)
ii. IRMA: immunoradiometrische analyse (=captrure ELISA)
c. Fluorescente groep
i. FIA (competitief en capture)
Bv. ISAC: Immuno Solid-phase Allergen Chip
d. Chemiluminescente groep
i. Chemiluminescentie

5. Gemerkte antilichamen (cellen)
a. Cellulaire assay (ELISA / FIA)
b. Flowcytometer

, 6. Gemerkte antilichamen (weefsel)
a. Directe methode
b. Tweestaps indirecte methode
c. Driestaps indirecte methode
d. Oplosbaar enzyme-immunocomplex (PAP – APPAAP)
e. (strept)avidine-biotine methodes
f. UltraAmp technologie
g. Dextraan polymeerketens
h. Biotynil-tyramide based detection systems



ONTWIKKELING T-LYMFOCYT
Beenmerg – multipotente stamcel  T-cel precursor


Thymus – thymocyten/T-celprecursor
1) Subcapsulaire schorszone
- Dubbel negatieve thymocyten CD4- en CD8-
- Geen TCR/CD3 complex: CD3-
STAP 1: vorming T-cel receptor (TCR)
- Onbeperkt aantal Ag
- Ag specifiek  ≠ in Vα en Vβ domein
- TCR = eiwit  gecodeerd door gen
! Genetische herschikking !

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