VET272 (with error-free answers)

How are enzymes classified? correct answers Specificity, Classification (By type of reaction) and Composition How does enzyme specificity work? correct answers It is controlled by structural and conformational complementarity (essentially how well it fits) between enzyme and substrate. Name three enzyme classifications based on type of reactions. correct answers Oxidoreductase, Ligase, Transferase. What is a holo-enzyme? correct answers A holo-enzyme is a complex enzyme composed of proteins and a relatively small organic molecule What is the name of the protein component of a holo-enzyme? correct answers Apo-enzyme What is the name of the non-protein component of a holo-enzyme? correct answers Prosthetic group. What is VFA? correct answers volatile fatty acids What is NEFA? correct answers Non esterified fatty acids 2 main sites of fat oxidation correct answers Adipose tissue and capillary walls 2 enzymes and which site correct answers Adipose tissue - Hormone sensitive lipase Lipoprotein lipase - capillary walls Function of Lipoprotein lipase correct answers LPL gets NEFA into cells. LPL active in fed state (insulin in adipose; low Km in muscle so always active with (substrate)) Function of Hormone sensitive lipase correct answers gets NEFA out of adipose tissue. HSL active in fasted state, when NEFA needs to be mobilised Oxidoreductase correct answers Catalyses transfer of electrons from electron donor to electron acceptor. Usually utilizes NADP+ or NAD+ as cofactors. Transferase: correct answers Transfers functional group from a donor substrate to an electron acceptor. Example are amine, carboxyl and methyl. Hydrolase correct answers Enzyme that catalyse the cleavage of a covalent bond using water. Examples are phosphatases or proteases. Lyase correct answers Breaks the bond via an elimination reaction of various chemical bonds rather than hydrolysis.