MB BOC EXAM PREP Q&A 2023

If you run a PCR product on a 0.8% agarose gel but the product shows nonspecific bands, how should you modify the next PCR reaction? a. reduce concentration of primers b. increase concentration of Taq polymerase c. increase the number of PCR cycles d. decrease the template denaturation temperature - Answer- a. reduce concentration of primers A reduction of primer concentration in a PCR reaction will result in an ____________ specificity of PCR - Answer- increased A confluent T25 flask of fibroblast cells is received for molecular genetic testing. The best way to handle this sample is to: a. accept it b. reject it c. incubate it at 45C d. freeze it at -70C - Answer- a. accept it Before reporting results, questionable or inconsistent data must be: a. resolved by additional analyses b. reported to the CQI committee c. submitted with the genotype report d. discarded according to CAP guidelines - Answer- a. resolved by additional analyses T/F: Questionable or inconsistent data should always be resolved prior to reporting - Answer- T Which of the following compositions of gels would allow for efficient separation of linear DNA molecules in the range of 100-300 base pairs in length? a. 0.3% agarose b. 1.0% agarose c. 1.0% polyacrylamide d. 8.0% polyacrylamide - Answer- d. 8.0% polyacrylamide When making gels, agarose is good for sizes _______- ___________ and polyacrylamide is good for sizes __________ - _________. - Answer- 200-50,000 200-1000