Microbiology practical 1Exam and Correct Solutions Rated A+

normal microbiota the microorganisms that are more or less permanant skin microbiome normal microbiota comprise this transient microbiota microbes that are present only for days or weeks microbiome the community that microbes that inhabit a particular environment chemically defined medium a medium whose exact chemical composition is known complex media -media for which the exact chemical composition varies slightly from batch to batch -media where more chemoheterotrophic bacteria are routinely grown nutrient broth a commonly used liquid complex medium nutrient agar when agar is added to nutrient broth agar an extract from marine red algae -has unique properties that make it useful in culture media steam sterilization/autoclaving -the most common method of sterilizing culture media that are heat stable -uses steam under pressure petri dish -where a bacteria could be grown -contains a solid media -provides a large surface area for examination of colonies inoculation -intentionally introduces incubation period the period during which the bacteria that are inoculated into culture media increase in number turbid -cloudy -liquid media looks this way as a result of bacterial growth after suitable incubation flocculent clumps of microbial cells pellicle a membrane across the surface of the broth fungi -possesses eukaryotic cells and can exist as unicellular or multicellular organisms -obtain nutrients through absorbing dissolved material through -aerobic yeast -nonfilamentous, multicellular fungi that are typically spherical/oval in shape pseudohypha a short chain of cells that forms when buds fail to detatch themselves molds multicellular filamentous fungi thallus a microscopic mold colony mycelium a mass of strands of mold hypha each individual strand of mold vegetative hyphae grow in or on the surface of the growth medium reproductive hyphae -aerial hyphae -originate from the vegetative hyphae and produce spores spores -asexual reproductive -produced from reproductive hyphae septum -a cross wall that separates the hyphal strand of most molds septate hyphae -the individual hyphae cells coenocytic hyphae -hyphae that lack septa and are a continuous mass of cytoplasm with multiple nuclei oomycota -fungus-like algae in the phylum (similar looks and growth requirements) zoospores motile asexual spores Zygomycota -saprophytic molds that have coenocytic hyphae -a phylum -ex) rhizopus (bread mold) saprophyte -obtains nutrients from dead organic matter -in healthy animals and plants -do not usually cause disease sporangium -asexual spore sac in Zygomycota -where asexual spores are formed sporangiospores asexual spores formed inside sporangium zygospores -sexual spores in Zygomycota -formed by fusion of 2 cells Ascomycota -molds w/ septate hyphae and some yeasts -called sac fungi -a phylum ex) penicillium and aspergillus ascospores -sexual spores in Ascomycota ascus -sac where ascospores are produced