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Lecture notes - Cell And Molecular Biology (DNA - Genes) - Using Becker's World of the Cell, Global Edition $8.51   Add to cart

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Lecture notes - Cell And Molecular Biology (DNA - Genes) - Using Becker's World of the Cell, Global Edition

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If you're studying a life science (e.g. - biomed, bioscience, physiology, sports science, sports physiology etc), then this detailed set of lecture notes on DNA and Genes will help you smash your first set of exams on cell/molecular biology! Try using this set of notes along with my other notes ...

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  • September 6, 2023
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  • 2019/2020
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Gene Regulation 21/10/19
- Gene regulation allows us to adapt to environmental change

All organisms must regulate which genes are expressed at a given time

Genes = turned off/on when responding to signals – from internal/ext. env.

Gene regulation = essential for cell specialization for multicellular organisms

Regulation differs in Bacterial cells and Eukaryotes

Bacterial regulation:
Bacterial cell that conserves energy/resources = advantage over one that can’t

- E.g. = E. coli – lives in Colon
- Needs AA tryptophan to survive
- If host person ≠ intake said AA – E. coli actives metabolic pathway – makes AA trypt. from
another compound
- If host person intakes trypt. – E. coli ≠ produce trypt. – stops using resources for metabolism

Metabolic pathway = controlled on 2 levels:

- Cells adjust activity of enzymes (catabolic enzyme activity = dependent on ‘chemical cues’)
- Activity of 1st enzyme in pathway = inhibited by pathway’s end product (trypt.)
- Accumulation of trypt. = inhibition of enzyme = no more trypt. production
- Process = feedback inhibition – lets cell adapt to short-term fluctuations of substance

- Cells adjust production of enzymes – regulate expression of genes coding for enzyme
- If trypt. ≠ needed – cell prevents production of enzymes catalysing synthesis of trypt.
- Process occurs at transcription level

Allosteric = Protein with structure altered reversibly by small molecule – modifying function



Many bacterial genes = switched on/off by metabolic changes in a cell

Operon model = basic mech. of gene expression control

, Operons:

Operon = cluster of functionally related genes – co-ordinately controlled by single ‘on/off switch’

- One promoter = sufficient for all 5 operon genes coding for enzymes for metabolic pathway
- One long mRNA strand = produced – all code for pp for enzymes
- Translation = separate due to presence of start/stop codons

Enzymes = simultaneously synthesised due to on/off switch

- On/off switch = segment of DNA (Operator)
- Controls access of RNA Polymerase to said genes

Operator, Promotor and genes being controlled = Operon

Operon = switched off by Repressor

- Binds to operator – blocks attachment of RNA pol. to promotor region
- Transcription of genes ≠ occur
- Rep. protein = specific for operator of certain operon
- Active or inactive form – determined by presence of other molecules

Rep. Protein = encoded by regulatory gene

- Expressed continuously (at low rate)
- Binding of repressors = reversible
- Operator = in 2 states – repressor bound and repressor unbound
- Repressor = allosteric – 2 shapes - active and inactive
- Inactive version = synthesized – low affinity for operator

Tryptophan = corepressor

- Small mol – binds to allosteric site of repressor – activates it
- More trypt. = more assoc. with repressor – less transcription of operon genes = less enzyme
- Less trypt. = less assoc. with repressor – more transcription of operon genes = more enzyme

Enzymes = repressible enzymes

Inducible Operons:

Inducible operons = usually off – stimulated/induced to be on when small molecule interacts with
different regulatory protein

- E.g. = lac operon (lactose)

Lac operon = genes coding for enzymes for hydrolysis & metabolism of lactose


- Lactose = available for E. coli – if host has milk
- Metabolism = hydrolysis of lactose to glucose and galactose

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