ASCP Mock Exam Questions & Answers 2023/2024
The derived linear range for a spectrophotometer is 0.0550 to 1.270. The 260 OD absorbance reading for a sample is 1.35. What is the appropriate action? - ANSWER-dilute the sample slightly and repeat the quantitation
Evaluation of a blood sample ...
The derived linear range for a spectrophotometer is 0.0550 to 1.270. The 260 OD absorbance reading for
a sample is 1.35. What is the appropriate action? - ANSWER-dilute the sample slightly and repeat the
quantitation
Evaluation of a blood sample indicates the presence of a clot. Corrective action to salvage this specimen
for analysis would most likely require - ANSWER-excess digestion of Proteinase K
An advantage of nitrocellulose membranes is that - ANSWER-results of colormetric reactions are more
intense
When performing allele specific oligonucleotide procedures, which techniques are generally employed? -
ANSWER-PCR, Autoradiography, Hybridization
Which of the following actions should NOT be done to a nylon membrane containing DNA after a
southern transfer? - ANSWER-Bake the membrane at 80C overnight
Calf intestinal phosphatase is used during the cloning procedure to - ANSWER-prevent vector re-ligation
Which of the following actions will bind DNA to a positively charged nylon membrane? - ANSWER-Baking
at 80C for 2 hours
Which of the following techniques is most appropriate for preparing high purity reagents for use in
cloning procedures - ANSWER-sterilizing through a 0.25 um disposable Millipore filter
Which of the following scenarios would best ensure fidelity of a cycle sequencing reaction - ANSWER-10-
25 uM dNTPs with greater than 55C annealing temperature
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