Understanding the biology of cells is essential for various fields, including medicine, genetics, and biotechnology. It allows scientists to delve into the mechanisms of life, develop treatments for diseases, and harness the power of cells for biotechnological applications. As we unravel the comple...
CELL FRACTIONATION :
Isolation of cellular components to
determining their structure's chemical composition is
called cell fractionation . It is a combination of various
procedures to separate cell organelles on the basis of “size”
&” density “.
It consists of two steps :
1_ Homogenization 2_ Sedimentation
HOMOGENIZATION:
Cell fractionation starts with
homogenization, Where large numbers of slag types of cells
break in an ice cold suitable medium with proper PH & ionic
composition. These cells are placed in homogenizer OR
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