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Molecular Diagnostics Fundamentals Methods and Clinical Applications 1st Edition by Lela Buckingham -Test Bank
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, Chapter 1: DNA1. Which of the following enzymes will untangle DNA?
A) Polymerase
B) Helicase
C) Kinase
D) Phosphatase
2. Which DNA sequence is homologous to 5GATTCTCAAAGGACT3?
A) 5GATTCTCAAAGGACT3
B) 3GATTCTCAAAGGACT5
C) 3CTAAGAGTTTCCTGA5
D) 5CTAAGAGTTTCCTGA3
3. Restriction endonucleases are enzymes that are produced by bacteria and __________
A) degrade viral proteins
B) degrade DNA
C) have no laboratory applications
D) degrade lipids
4. In a DNA molecule, the 5 end contains a free ___________
A) phosphate group
B) hydroxyl group
C) amino group
D) carboxyl group
5. The term used to describe the arrangement of the individual strands in the double-stranded
DNA molecule is:
A) parallel
B) antiparallel
C) tangential
D) none of the above
6. One DNA strand binds to the other strand through:
A) peptide bonds
B) disulfide bonds
C) hydrogen bonds
D) phosphodiester bonds
7. DNA replication is:
A) conservative
B) semiconservative
C) nonconservative
D) dispersive
Page 1
, Chapter 1: DNA
8. In a double-stranded DNA molecule, base-pairing between strands occurs between:
A) a purine and a purine
B) a pyrimidine and a pyrimidine
C) a purine and a pyrimidine
D) any type of nucleotide base
9. DNA replication requires the presence of:
A) DNA template
B) RNA polymerase
C) amino acids
D) messenger RNA
10. The process of separating the two DNA strands into two single strands is called:
A) denaturation
B) bidirectional
C) depolymerization
D) synthesis
11. DNA replication proceeds with the new daughter strand synthesized in the following
orientation:
A) 5 to 3
B) 3 to 5
C) The daughter strand is not synthesized in any order
D) Either 5 to 3 or 3 to 5
12. The lagging DNA strand is synthesized discontinuously producing:
A) Kornberg fragments
B) Southern fragments
C) Okasaki fragments
D) Klenow fragments
13. In DNA replication, where does replication begin and end?
A) Origin of replication; end of the molecule
B) Promoter; termination site
C) Start codon; stop codon
D) None of the above
14. An exon is:
A) spliced out
B) an open reading frame (ORF)
C) a DNA-binding protein
D) within 100 bp of ORFs
Page 2
, Chapter 1: DNA
15. The nucleotides in a single strand of DNA are held together by which of the following
bonds?
A) Disulfide
B) Phosphodiester
C) Hydrogen
D) Peptide
16. In DNA replication, the leading strand is the strand that has which conformation?
A) 5 to 3
B) 3 to 5
C) Both strands are leading
17. Which of the following is a purine?
A) Thymine
B) Cytosine
C) Adenine
D) Alanine
18. Which of the following does not play a role in DNA replication?
A) RNA primer
B) Deoxynucleotide
C) Stop codon
D) DNA polymerase
19. The purpose of endonucleases is to:
A) synthesize DNA
B) cut DNA within the double helix
C) chew DNA from the ends
D) paste two ends of DNA together
20. Nucleotides in DNA consist of which of the following?
A) Nitrogen base, deoxyribose, and phosphate
B) Nitrogen base, ribose, and sulfur
C) Carbon base, ribose, and phosphate
D) Carbon base, glucose, and carboxyl
21. Adenine, thymine, guanine, and cytosine are what components of DNA?
A) Hydrogen bonds
B) Sugar moieties
C) Phosphodiester groups
D) Nitrogen bases
Page 3
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