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MEDIA REACTIONS MIBO PRACTICE EXAM QUESTIONS AND ANSWERS(DIAGRAMS INCLUDED)
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MEDIA REACTIONS MIBO PRACTICE EXAM QUESTIONS AND ANSWERS(DIAGRAMS INCLUDED)
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MEDIA REACTIONS MIBO PRACTICEEXAM QUESTIONS AND
ANSWERS(DIAGRAMS INCLUDED)
Selective and Differentials
MSA, BP, MAC, EMB
MAC Ecoli and S. marceesans
MacConkey agar plate inoculated with the gram-negative lactose fermenter Escherichia coli and
the gram-negative non-lactose fermenter Serratia marcescens.
MAC acid
pink - lac pos
MAC Neutral to alkaline
Opaque--lac negative
MAC Ecoli and another weaker fermenter
MacConkey agar plate inoculated with Escherichia coli using the streak plate technique
Weak fermenter= Entero aerogenes
MSA ferment
Mannitol salt agar inoculated with Staphylococcus aureus showing mannitol fermentation
(yellow medium)
, MSA ferment and no growth
Mannitol salt agar inoculated with S. aureus showing fermentation of mannitol (yellow medium)
on one side and inoculated with Streptococcus durans showing no growth on the other side
MSA growth/ no ferment
Mannitol salt agar inoculated with Micrococcus luteus showing no fermentation of mannitol
(pink medium)
MSA Staph Citreus
Mannitol salt agar inoculated with Staphylococcus citreus showing fermentation of mannitol
(yellow medium)
MacConkeys general
Selective: CV and Bile salts (selects AGainst G+)
Differential: neutral red as pH indicator for lactose
Mannitol Salt Agar general
Selective: NaCl (selects for Staph)
Differential: phenol red (pH) for mannitol (yellow = mannitol ferment)
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