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MIBO 3500L FINAL EXAM WITH 100% CORRECT ANSWERS 2023
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MIBO 3500L FINAL EXAM WITH 100% CORRECT ANSWERS 2023
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MIBO 3500L FINAL EXAM WITH 100%CORRECT ANSWERS 2023
Culture
A nutrient medium growing one or more species of microorganisms
Pure culture (anexic)
When a culture contains only one species of microorganism
-Simplifies experimental design and interpretation of results
-Must be made up of individual cells of a single genetic makeup (genotype)
-A colony is usually derived from a single cell and thus can be used to inoculate medium and
generate this
Wire (platinum) inoculating loop
Used to transfer microbes from one culture medium to another, expedites sterilization and
inoculation processes because:
-Conducts heat extremely well
-Cools just as rapidly as it acquires heat
Plastic inoculating loop
Used to transfer microbes from one culture medium to another
-Not flamed before use
-Pre-sterilized and disposable
Inoculating wooden stick
Used to transfer microbes from one culture medium to another
-Pre-sterilized
-Reusable
-Not flamed before use
TSBYE tube inoculations
1. Hold sterilized transfer instrument (either loop or stick) in your dominant hand
2. Hold the bacterial culture in your non-dominant hand
3. Remove the cap of the bacterial culture without touching your transfer instrument to
, anything
-One way to do this is to curl the little finger of your dominant hand around the cap and twist it
off
4. Dip the transfer instrument into the bacterial culture, then remove the instrument and
replace the cap on the culture tube
-Work carefully but quickly; the longer the tube is open the higher the risk of contamination
from airborne microbes...only uncap the tube when you are ready to use it
5. While still holding the transfer instrument, place the bacterial culture back in the test tube
rack and pick up the TSBYE tube
6. Remove the lid of the TSBYE tube the same way you did in step 4
7. Dip the transfer instrument containing bacteria into the TSBYE tube, then remove the
instrument and replace the cap on the tube
-This will inoculate the TSBYE medium with the bacteria from the transfer instrument
8. Set the newly inoculated TSBYE tube back in the test tube rack and discard your transfer
instrument in the appropriate waste container
9. Inoculate your second tube as a "mock control" (no bacteria)
10. Your TA will inoculate your tubes at 37°C for 24 hours then store them for you to view next
class
11. Observe the TSBYE tubes for turbidity (cloudiness), which is an indication of bacterial growth
TSAYE slant inoculations
1. Hold sterilized transfer instrument (either loop or stick) in your dominant hand
2. Hold the bacterial culture in your non-dominant hand
3. Remove the cap of the bacterial culture without touching your transfer instrument to
anything
-One way to do this is to curl the little finger of your dominant hand around the cap and twist it
off
4. Dip the transfer instrument into the bacterial culture, then remove the instrument and
replace the cap on the culture tube
-Work carefully but quickly; the longer the tube is open the higher the risk of contamination
from airborne microbes...only uncap the tube when you are ready to use it
5. While still holding the transfer instrument, place the bacterial culture back in the test tube
rack
6. Pick up the TSAYE slant with your non-dominant hand and remove the lid the same way you
did in step 4
7. Place the transfer instrument flat against the agar and gently move the instrument in a zigzag
motion up the slant from bottom to top
8. Set the newly inoculated TSAYE slant back in the test tube rack and discard your transfer
instrument in the appropriate waste container
9. Inoculate your second slant as a "mock control" (no bacteria)
10. Your TA will inoculate your tubes at 37°C for 24 hours then store them for you to view next
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