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Short Tandem Repeats

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This presentation provides knowledge on STRs, their structure, classification, appearance on a profile and comes with attached flashcards with more information on artefacts that may be present in a DNA profile. This information was obtained from my lecture notes from university in the Forensic Ge...

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  • November 18, 2023
  • 13
  • 2023/2024
  • Class notes
  • Judith smith
  • Short tandem repeats
  • Unknown
  • Unknown
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Available practice questions

Flashcards 14 Flashcards
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Some examples from this set of practice questions

1.

STR

Answer: Short Tandem Repeat/ microsatellites. 1-6bp long

2.

Fluorescence Detection

Answer: Dye molecule is excited by a laser. The dye emits light which can then be detected.

3.

Labelling DNA

Answer: 3 methods: -Labelling 5\' end of one primer -Incorporate labelled dNTPs into the product -Use a fluorescent intercalating dye

4.

Collection Software Conditions

Answer: Voltage, injection time, injection amount

5.

GeneMapper

Answer: Processes raw data and displays the data used to assign alleles to the DNA profile.

6.

Interpretation of the Peaks

Answer: Interpret artefacts, peak balance, peak morphology and peak height/peak area ratio.

7.

Stutter peaks

Answer: A peak one repeat less than the real allele because of mispairing in the PCR reaction. Affects dinucleotides the most because they\'re shorter. To solve- use complex repeats

STRs
Structure + Use

, WHY USE STRs?

• VNTRs alleles are hard to analyse and are too long



• ampFLPs such as DS180 are too polymorphic and too
big



• STRs are quite polymorphic and have identifi able and
discrete alleles

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