This presentation provides knowledge on STRs, their structure, classification, appearance on a profile and comes with attached flashcards with more information on artefacts that may be present in a DNA profile.
This information was obtained from my lecture notes from university in the Forensic Ge...
University of Central Lancashire Preston (UClan)
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Available practice questions
STRs and DNA Profile Artefacts
Flashcards14 Flashcards
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Flashcards14 Flashcards
$3.910 sales
Some examples from this set of practice questions
1.
STR
Answer: Short Tandem Repeat/ microsatellites. 1-6bp long
2.
Fluorescence Detection
Answer: Dye molecule is excited by a laser. The dye emits light which can then be detected.
3.
Labelling DNA
Answer: 3 methods:
-Labelling 5\' end of one primer
-Incorporate labelled dNTPs into the product
-Use a fluorescent intercalating dye
4.
Collection Software Conditions
Answer: Voltage, injection time, injection amount
5.
GeneMapper
Answer: Processes raw data and displays the data used to assign alleles to the DNA profile.
6.
Interpretation of the Peaks
Answer: Interpret artefacts, peak balance, peak morphology and peak height/peak area ratio.
7.
Stutter peaks
Answer: A peak one repeat less than the real allele because of mispairing in the PCR reaction. Affects dinucleotides the most because they\'re shorter.
To solve- use complex repeats
Content preview
STRs
Structure + Use
, WHY USE STRs?
• VNTRs alleles are hard to analyse and are too long
• ampFLPs such as DS180 are too polymorphic and too
big
• STRs are quite polymorphic and have identifi able and
discrete alleles
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