ASCP Recalls Subject: Microbiology graded A+

Identify the organism: Bile esculin: POSITIVE; 6.5% NaCl: POSITIVE; PYR: NEGATIVE; LAP: NEGATIVE Ans: Leuconostoc 2. A gram stain from a blood agar shows gram positive cocci in chains, non-hemolytic, The bacteria grow on 6.5% NaCl and is Bile esculin positive. What’s the most likely identification? Ans: Enterococcus ( only Enterococcus can grow both 6.5% NaCl and Bile Esculin ) 3. latex agglutination for Staphylococcus aureus: Ans: Protein A and clumping factor 4. Pink mucoid colonies on MACTSI=A/A Spot indole test: POSITIVE Ans: Klebsiella oxytoca 5. A patient is diagnosed wit streptococcal pharyngitis 6 months ago. Now he is showing signs of glumerulonephritis. What will be seen in the renal biopsy? Ans: S. pyogenes ( S. pyogenes is the most common causative agent for pharyngitis) 6. Biochemical reaction: Bile esculin: positive; 6.5% NaCl: negative Ans: Streptococcus bovis ( 6.5% NaCl test along with Bile-Esculin test, is used in many laboratories to distinguish Enterococcus species from the group D streptococci, Streptococcus bovis and Streptococcus lactis ) 7. Which of the following tests cab differentiate Pseudomonas aeruginosa from Pseudomonas putida? Ans: Growth at 42 degrees Celsius ( P. aeruginosa had the widest range of growth temperature 25-42°C ) 8. Favours growth of anaerobic gram negative bacilli: Ans: Vitamin K and hemin 9. which of the following tests should be done first in order to differentiates Aeromonas spp from the Enterobacteriaceae? Ans: Oxidase ( Aeromonas: TSI reaction: A/A, and is Oxidase Positive. Enterobacteriaceae is oxidase Negative )