MB (ASCP) Exam Questions and Answers Graded A+
Predominant form of DNA Right-handed B
DNA and histone interaction is due to what type of bond? Ionic
What stains to the minor groove of DNA duplex? SYBR Green
What is the most abundant type of RNA? Ribosomal RNA (rRNA)
What does RNA act as in...
MB (ASCP) Exam Questions and Answers Graded A+
Predominant form of DNA ✔✔ Right-handed B
DNA and histone interaction is due to what type of bond? ✔✔ Ionic
What stains to the minor groove of DNA duplex? ✔✔ SYBR Green
What is the most abundant type of RNA? ✔✔ Ribosomal RNA (rRNA)
What does RNA act as in a retrovirus? ✔✔ Genome and mRNA
What increases half-life of mRNA? ✔✔ 5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active? ✔✔ Euchromatin
What is the most common type of eukaryotic DNA? ✔✔ Satellite DNA
What specimen has the most DNA according to molecular weight? ✔✔ White Blood
Cells
What amino acids compose histones? ✔✔ Lysine and Arginine
Amino acid that all proteins start with: ✔✔ Methionine
What protects the DNA strand during replication? ✔✔ Single-stranded binding protein
Leading Strand ✔✔ 3' to 5' template
Lagging Strand ✔✔ 5' to 3' template
What DNA strand is being copied in transcription? ✔✔ Antisense Strand
First step after newly formed mRNA is made? ✔✔ Addition of poly(A) tail
Every time the chromosome is copied it would become shorter if not for what enzyme?
✔✔ Telomerase
What polymerase has repair activity? ✔✔ Beta/Epsilon
What polymerase acts on leading strand? ✔✔ Delta
What polymerase acts on lagging strand? ✔✔ Alpha
, Which polymerase is used for mitochondrial replication and repair? ✔✔ Gamma
What polymerase binds the 5' end to 3' end of dsDNA? ✔✔ Topoisomerase
Which RNA polymerase is mainly for transcription? ✔✔ RNA Pol II
Which polymerase is involved with mRNA synthesis? ✔✔ RNA Pol II
What inactivates RNAses? ✔✔ Diethyl pyrocarbonate (DEPC) or bake glassware for 4
to 6 hours at 400C
What type of restriction enzyme recognizes palindromic sites? ✔✔ Restriction Enzyme
Type II
What type of restriction enzyme lacks methylation activity? ✔✔ Restriction Enzyme
Type II
What is necessary to make cDNA? ✔✔ Reverse transcriptase
Number of SNPs in a human genome? ✔✔ 11 billion (10^7)
How many genes (base pairs) are in a human haploid genome? ✔✔ 3 billion base pairs
The polymerization of acrylamide requires: ✔✔ Catalyst (APS or TEMED) or Light
activation
How does agarose gel pore size relate to concentration? ✔✔ Inversely proportional
What is denatured during a southern blot? ✔✔ Hydrogen bonds (inter-strand bonds)
Melting temperature formula ✔✔ Tm = (4C x GC Pairs) + (2C x AT pairs)
Which is likely to interfere with fluorescent probe? ✔✔ 5' G
Proper GC content of primer ✔✔ 35-65%
Inhibitors of PCR ✔✔ Hemoglobin, Heparin, Xylene, Phenol, Cyanol, and Bromophenol
Blue
Order to add to a PCR reaction: ✔✔ Master mix > patient sample > positive control >
negative control
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller ngnstuvia. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $19.69. You're not tied to anything after your purchase.
