COMPLETE TEST BANK: Fundamentals of Molecular Virology 2nd Edition by Nicholas H. Acheson Latest Version.
Test Bank for Fundamentals of Molecular Virology 2nd edition by Acheson, ISBN: 9780470900598, All 37 Chapters Covered | Complete Solution Guide.
Fundamentals of Molecular Virology 2nd edition by Acheson - Test Bank, All 37 Chapters Covered, Verified Latest Edition
All for this textbook (13)
Written for
Wageningen University (WUR)
MSc Biotechnology
Molecular Virology (VIR30306)
All documents for this subject (3)
Seller
Follow
célinebrouwerswur
Content preview
Lecture 1 - Introduction
Virus
= genetic entities (RNA or DNA) protect by protein coat , sometimes also a lipid membrane
which uses the synthesizing system of host witch has pathological effects on the host cell
all organisms have
viruses
Pathogens smaller than virus:
- Viroid = only RNA, circular, disease making
- Prions = disease making proteins, misformed
Composition of a virus
- viral genome (RNA or DNA)
- protein shell, made up from CPs
= coat proteins
- lipid membrane / envelope
- GP = envelope glycoprotein
Baltimore classification
= Classification based on how viruses make
+mRNA from their genomic material.
-ssRNA → viral RNA polymerase needed
Protein coat
Why subunits: coat proteins (CPs)
- saving genetic space on small genome
- increasing genetic stability (smaller gene → lower risk for mutation)
- easy (dis)assembly
- symmetric → minimal energy & less visible for immune system
,Function protein coat
- protection of genetic material
- recognition and penetration of the host cell
Structure
The higher the fold, the more spherical the protein coat will be
T=3 icosahedral capsid protein T=16 icosahedral capsid protein
pentameres = 12 asymmetric units = 5*12 = 60 capsid proteins = 60*T
T=triangulation, the higher the T → bigger particle
because capsid proteins are the same size
Entry of virus into the cell
1. Attachment
2. (internalization in endosomes)
3. Fusion
4. Transport
,Lecture 2 - Plant viruses (+mRNA)
The eukaryotic protein translation system Plant viral ss(+) RNA genome organization
The plant translational machinery only multiple ORFs
translate monocistronic messengers
(mRNAs) = one open reading frame (ORF)
ORF1 replication
(polymerase, helicase, methyl transferase)
ORF2 movement
UTR = untranslated region ORF3 encapsidation (coat proteins)
viral mRNA needs to mimic the eukaryotic mRNA
Strategies to overcome differences in mRNA:
1. Segmentation one ORF per mRNA
2. Subgenomic one ORF mRNA split of from
RNAs the main mRNA
3. Read-through only part of mRNA is
translation transcribed due to weak
start/stop codon
4. Frame shifting ribosome shifts to another
ORF so not all ORF translated
5. Polyprotein transcribed as one protein,
then processed into multiple
, Potato virus Y (PVY) Potato leafroll virus (PLRV)
5. Polyprotein
part of polyprotein can be protease → cuts
others (trans) or itself (cis)
2. Subgenomic RNAs
3. Read-through translation
4. Frame shifting
5. Polyprotein
Infection cycle
Virus entrance
Mechanical rupture in the cuticle caused by:
- Piercing → aphid, whitefly, thrips, nematode
- Biting → beetle
- Handling of plants
no receptor-mediated endocytosis like with animal-infecting viruses
Replication & translation
First translate protein needed for
replication
→ co-translational disassembly
Then +ssRNA → -ssRNA → +ssRNA
→ co-replicational disassembly
Translation of
movement/encapsidation proteins
Then assembly of new viruses
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller célinebrouwerswur. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $8.99. You're not tied to anything after your purchase.
