ASCP MB EXAM UPDATE 2023/2024 NEW UPDATE/ ASCP MB EXAM REAL EXAM WITH QUESTIONS AND VERIFIED SOLUTIONS
2 views 0 purchase
Course
ASCP MB
Institution
ASCP MB
ASCP MB EXAM UPDATE 2023/2024 NEW
UPDATE/ ASCP MB EXAM REAL EXAM WITH
QUESTIONS AND VERIFIED SOLUTIONS ASCP MB EXAM UPDATE 2023/2024 NEW
UPDATE/ ASCP MB EXAM REAL EXAM WITH
QUESTIONS AND VERIFIED SOLUTIONS ASCP MB EXAM UPDATE 2023/2024 NEW
UPDATE/ ASCP MB EXAM REAL EXAM WITH
QUESTIONS A...
ASCP MB EXAM UPDATE 2023/2024 NEW
UPDATE/ ASCP MB EXAM REAL EXAM WITH
QUESTIONS AND VERIFIED SOLUTIONS
What genes would be screened in a lung cancer panel? - ANSWERS-KRAS, EGFR,
ALK
In a retrovirus, the RNA acts as: - ANSWERS-Genome and mRNA
To what does the AcrAB gene product create resistance to? - ANSWERS-Tetracycline
What gene is measured following the treatment with imatinib(Gleevec)? - ANSWERS-
BCR/Abl
Blood drawn into what type of tube is compatible for genetic testing? - ANSWERS-K2-
EDTA tube
Primer dimers are due to what complimentary issue? - ANSWERS-3' to 3'
complementarity of primer pairs
Microsatellite instability is best described as: - ANSWERS-Contraction or expansion of the
genomes caused by frameshift mutations (deletions or insertions) in elements of the genome
consisting of a repeating sequence of 1-3 base pairs
How many hydrogen bonds are formed between one C:G base pair? - ANSWERS-3
When this enzyme finds nicks or gaps in the sugar-phosphate backbone of DNA, it
closes them - ANSWERS-Ligase
,What assay amplifies the target using DNA ligase? - ANSWERS-LCR
What genes would be screened in a breast cancer panel? - ANSWERS-HER2, ERBB2,
BRCA1
What factors can affect nucleic acid hybridization? - ANSWERS-G:C ratio of bases
pH of the hybridization reaction
Hybridization temperature
Both parents are carriers of an autosomal recessive disorder. What percent chance do
these parents combined have to pass down this affected gene to their child? -
ANSWERS-75%
Consider the probe sequence, CTACCGTAATATTCGACCGT, to be used in a hybridization
procedure. What is the melting temperature, Tm, of the sequence? -
ANSWERS-58*C
The enzyme that primes DNA synthesis is: - ANSWERS-DNA primase
Splicing is the process that: - ANSWERS-Removes introns and preserves exons
All of the following enzymes are part of the DNA replication machinery - ANSWERS-
Ligase, DNA polymerase, Helicase
This translocation creates a fusion protein between the Abl1 gene on one chromosome
and the BCR gene on the other chromosome, resulting in chronic myelogenous
leukemia (CML): - ANSWERS-t(9;22)
Given the anti-sense strand of DNA: 5'-AATTGCCGACATAGAT-3' which is the
appropriate sense strand? - ANSWERS-5'-ATCTATGTCGGCAATT-3'
,All of the following methods of amplification are considered target amplification: - ANSWERS-
Quantitative PCR
Strand Displacement Amplification
Reverse Transcriptase PCR
What stain binds to minor groove of DNA Duplex? - ANSWERS-SYBR1
The purines are: - ANSWERS-Adenine and guanine
Purines and pyrimidines differ from each other in that: - ANSWERS-Purines have two
rings; pyrimidines have one ring
What gene is measured following treatment with Warfarin? - ANSWERS-VKORC1
According to Chargaff's rule of base pairing, adenine pairs with: - ANSWERS-Thymine
When comparing 2 samples in a qPCR Amplification plot, a log difference can be seen
as approximately: - ANSWERS-ΔCt = 3.3
The process of making RNA is termed: - ANSWERS-Transcription
When sequencing HLA-DR what is targeted? - ANSWERS-Exon 2 of the β subunit
All of the following are components of nucleic acids: - ANSWERS-Phosphate group
Sugar (ribose or deoxyribose)
Nitrogenous base (A,C,G,T)
Branched DNA amplification (bDNA) is best classified as: - ANSWERS-Signal
amplification
This PCR method works by generating a signal at the annealing step (i.e. when the
, probe binds its target) of the PCR reaction: - ANSWERS-Molecular beacons
What is the predominant form of DNA? - ANSWERS-B form
Mantle cell lymphoma (MCL) is caused by what translocation? - ANSWERS-t(11;14)
What responds poorly to Ribavarin/Interferon treatment? - ANSWERS-HCV type 1 & 4
The sugar in DNA is: - ANSWERS-Deoxyribose
Why is it critical to maintain workflow from "clean" to "dirty" while working in a molecular
lab? - ANSWERS-To avoid contamination of specimens with amplified product which
create false positive results
Which two HPV types are responsible for most cases of cervical cancer? - ANSWERS-
16 and 18
The amelogenin locus, found on the sex chromosomes, is used in gender identification.
Amplification at this locus reveals 2 peaks of sizes 212 and 218 base pairs. If this amplification
were part of a gender identification procedure, what would be the gender of the individual? -
ANSWERS-Male
This restriction enzyme "digests and removes a methyl group from Adenine": -
ANSWERS-Type I
To what does the mecA gene product create resistance to? - ANSWERS-Penicillin
3 cerebrospinal fluid (CSF) tubes labeled 1 through 3 have arrived in your clinical laboratory for
evaluation. The tubes were numbered in the order in which they were obtained, with #1 being the
first tube collected and #3 being the last tube collected.
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller brendawambui0309. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $21.49. You're not tied to anything after your purchase.
