#O C R
GCSE (9–1) Biology B (Twenty First Century Science)
J257/04 Depth in Biology (Higher Tier)
June 2023
Time allowed: 1 hour 45 minutes
H
You must have:
a ruler (cm/mm)
You can use:
an HB pencil
a scientific or graphical calculator
Please write clearly in black ink. Do not write in the barcodes.
Centre number Candidate number
First name(s)
Last name
INSTRUCTIONS
• Use black ink. You can use an HB pencil, but only for graphs and diagrams.
• Write your answer to each question in the space provided. If you need extra space use
the lined pages at the end of this booklet. The question numbers must be clearly shown.
• Answer all the questions.
• Where appropriate, your answer should be supported with working. Marks might be
given for using a correct method, even if your answer is wrong.
INFORMATION
• The total mark for this paper is 90.
• The marks for each question are shown in brackets [ ].
• Quality of extended response will be assessed in questions marked with an asterisk (*).
• This document has 24 pages.
ADVICE
• Read each question carefully before you start your answer.
2023 Turn over
, 2
1 A scientist cuts a thin slice from a plant stem, as shown in Fig. 1.1.
The slice of stem contains three different tissues.
Fig. 1.1
Tissue B
Tissue A
Plant stem
Tissue C
(a) Draw lines to connect the description of each tissue with its correct name.
One has been done for you.
Description Name
Tissue A
transports water from the Capillary
plant’s roots
Meristem
Tissue B
is made of unspecialized cells
Phloem
Tissue C
transports sugar from
Xylem
the plant’s leaves
[1]
2023
, 3
(b) The scientist places the slice of stem on a microscope slide and adds a cover slip.
The scientist uses the light microscope in Fig. 1.2 to look at the slice of stem on the slide.
Fig. 1.2
Eyepiece lens
Coarse focus
control
Objective lenses
Fine focus
Stage control
Mirror
(i) Statements A to E describe steps the scientist uses to look at the slide under the
microscope.
They are not in the correct order.
A Place the slide on the stage.
B Look into the eyepiece lens.
C Use the coarse focus control to move the objective lens down towards the stage.
D Use the fine focus control to move the objective lens until the image is as clear as
possible.
E Use the coarse focus control to move the objective lens upwards until the image is
clear.
Write the letters in the boxes to describe the correct order of the steps.
One has been done for you.
A
[3]
(ii) Suggest two ways the scientist can work safely when using the microscope.
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