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Samenvatting humane biologie I leerjaar 2 HLO
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Samenvatting humane biologie leerjaar 2 Hogeschool Arnhem/Nijmegen
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Samenvatting humane biologie I les 1MICROSCOPIE
lichtmicroscopie
- Bright fiel (giwoni)
- Feuoriscinti
- Fasi-contrast
- Confocaae
- Poearisirinl
3 einzin in iin eichtmicroscoop:
conlinsor,objictif,ocueair > aeei van geas
functi: virgrotng
hogiri risoeuti, bieangrijksti eins Ļ objictif
Condensor: bunlieing eicht
oculair: ook virgrotng
Doel: iin zo hielir mogieijk biiel virkrijgin mit litaies.
risoeuti > kiinsti afstanl lat ji 2 puntin gischiilin van iekaar
kunt waarnimin.
Virbitirin: hogiri virgrotng, bitiri risoeuti
köheirs instieeing Ļ instieeing microscoop corrict instieein.
Resolutie bepaald door:
1) numiriiki apirtuur
kwaeitiit objictif eins (Numiriiki apirtuur, NA)
hoi hogir NA, hoi miir eicht vanuit priparaat kan worlin
opgivangin > miir litaies.
2 )goeflingti
iin objict lat ongiviir li hief zo groot is aes li goeflingti kan
worlin ongischiilin.
zichtbaar eicht: goeflingti 400-700 nm
risoeuti: ca. 2 00nm
Risoeuti bipaael loor numiriiki apirtuur goeflingti.
R Ļ eata / 2 NA
max NA. Ļ 1.35
1
, conlinsor: apirtuur liafragma. Moit lus voor iilir objictif worlin aangipast.
Electronenmicroscopie
- Transmissii
- Scanning
Atomic forci microscopy
Risoeuti tot 1 nm
gibruikt ieiktronin (mit iin viei keiiniri goeflingti lan eicht)
hoi worlin li ieiktronin gibunliel? Door li eins magnitin. Eeiktromagnitin.
ATOMIC FORCE MICROSCOPY
2
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