BIO
110
Lab
Practical
Practice
1.
Which
of
the
following
distinguishes
the
Archaea
from
the
diplomonads?
A.
The
diplomonads
have
membrane-bound
nuclei,
but
Archaea
do
not.
B.
The
Archaea
have
a
membrane-bound
nucleus,
but
diplomonads
do
not.
C.
The
diplomonads
are
prokaryotic,
but
the
Archaea
are
eukaryotic.
D.
The
diplomonads
are
eukaryotic,
but
the
Archaea
are
prokaryotic
E.
Both
A
and
D.
-
ANSWER-E.
Both
A
and
D.
2.
Please
note
the
"Organism
List"
below.
What
feature
do
the
Protists
listed
there
hold
in
common?
A.
All
are
photosynthetic.
B.
All
are
a
type
of
cyanobacterium.
C.
All
contain
chlorophyll
d.
D.
All
are
multicellular.
E.
All
contain
alveoli
just
beneath
their
plasma
(cell)
membranes.
Organism
List
1.
Eubacteria
2.
Archaea
3.
Fungi
-
Phylum
Zygomycota
4.
Fungi
-
Phylum
Basidiomycota
5.
Protists
-
Plasmodium
6.
Protists
-
Paramecia
7.
Protists
-
Peridinium
-
ANSWER-E.
All
contain
alveoli
just
beneath
their
plasma
(cell)
membranes.
3.
Note
the
protists
below.
Which
property
below
would
best
split
them
into
two
groups?
A.
Nuclear
status,
i.e.,
eukaryote
versus
prokaryote
B.
Nutritional
type,
i.e.,
photosynthetic
versus
non-photosynthetic
C.
Cell
wall
type,
i.e.,
some
of
the
protists
listed
have
chitin
cell
walls,
some
do
not. D.
Mobility,
i.e.,
pseudopodia
versus
flagella
E.
Presence
of
kinetoplasts,
i.e.,
some
of
the
protists
listed
have
kinetoplasts,
some
do
not.
Organism
List
1.
Eubacteria
2.
Archaea
3.
Fungi
-
Phylum
Zygomycota
4.
Fungi
-
Phylum
Basidiomycota
5.
Protists
-
Radiolaria
6.
Protists
-
Paramecia
7.
Protists
-
Peridinium
-
ANSWER-B.
Nutritional
type,
i.e.,
photosynthetic
versus
non-photosynthetic
(Peridinium
is
photosynthetic,
the
others
are
not.)
4.
Note
the
"Organism
List"
below.
Which
property
below
would
best
split
the
first
two
groups
from
the
rest?
A.
Nuclear
status,
i.e.,
eukaryote
versus
prokaryote
B.
Nutritional
type,
i.e.,
autotroph
versus
heterotroph
C.
Cell
wall
type,
i.e.,
the
first
two
listed
have
chitin
cell
walls,
the
others
do
not.
D.
Mobility,
i.e.,
pseudopodia
versus
flagella
E.
Presence
of
ribosomes,
i.e.,
the
first
two
listed
have
ribosomes,
the
others
do
not.
Organism
List
1.
Eubacteria
2.
Archaea
3.
Fungi
-
Phylum
Zygomycota
4.
Fungi
-
Phylum
Basidiomycota
5.
Protists
-
Radiolaria
6.
Protists
-
Paramecia
7.
Protists
-
Peridinium
-
ANSWER-A.
Nuclear
status,
i.e.,
eukaryote
versus
prokaryote
The
first
two
groups
are
prokaryotes.
5.
Which
of
the
following
is
true
of
protists?
A.
All
protists
are
prokaryotic. B.
Protists
are
eukaryotes
that
cannot
be
classified
as
fungi,
plants
or
animals.
C.
Protists
are
a
microscopic
form
of
fungi.
D.
Protists
are
a
microscopic
form
of
plants.
E.
Protists
are
a
microscopic
form
of
animals.
-
ANSWER-B.
Protists
are
eukaryotes
that
cannot
be
classified
as
fungi,
plants
or
animals.
6.
In
the
electrophoresis
lab,
which
of
the
following
best
represent(s)
variables
that
you
manipulated
(independent
variables)?
A.
The
amount
of
protein
added
to
the
agarose
to
cause
it
to
solidify.
B.
The
pH
of
the
buffer
used
to
prepare
the
agarose
gels.
C.
The
voltage
applied
to
the
gel
as
it
cooled.
D.
The
maximum
temperature
of
the
agarose
as
it
boiled.
E.
The
distance
each
protein
migrated
in
an
electric
field.
-
ANSWER-B.
The
pH
of
the
buffer
used
to
prepare
the
agarose
gels.
7.
Which
of
the
following
correctly
characterize
the
isoelectric
point
of
a
protein?
A.
It
is
the
pH
at
which
the
net
charge
on
the
protein
is
negative.
B.
It
is
the
pH
at
which
the
net
charge
on
the
protein
is
positive.
C.
It
is
the
pH
at
which
the
protein
will
not
migrate
in
an
electrical
field.
D.
It
is
the
pH
at
which
the
protein
will
migrate
toward
the
positive
electrode.
E.
It
is
the
environmental
pH
at
which
the
protein
will
migrate
toward
the
negative
electrode.
-
ANSWER-C.
It
is
the
pH
at
which
the
protein
will
not
migrate
in
an
electrical
field.
This
is
true
because
the
protein
will
have
no
net
electrical
charge
at
its
isoelectric
pH,
so
it
will
not
be
influenced
by
an
electrical
field.
8.
A
corn
protein
known
to
contain
a
considerable
amount
of
lysine
and
very
little
aspartic
acid
or
glutamic
acid
is
placed
into
a
very
low
pH
solution.
Knowing
that,
which
of
the
following
is
most
likely
to
prove
true?
A.
At
that
low
pH
the
protein
will
have
a
net
positive
charge.
B.
At
that
low
pH
the
protein
will
have
a
net
negative
charge.
C.
At
that
low
pH
the
protein
will
have
a
net
zero
charge.
D.
At
that
low
pH
the
protein's
isoelectric
point
will
also
be
low.
E.
At
that
low
pH
the
protein's
isoelectric
point
will
increase.
-
ANSWER-A.
At
that
low
pH
the
protein
will
have
a
net
positive
charge.
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