ACS Biochemistry First Semester Exam With Complete Solutions 2024

ACS Biochemistry First Semester Exam With Complete Solutions 2024 Buffer Order and Henderson Hasselbach - answerWater, Buffer, Salt, Base pH = pKa + log(A/HA) pKa decrease as temp increase so pH decrease Ramachandran plots - answerΦ = N-R ψ = C-R optimal angle to prevent steric hinderance Protein structure of a helix, B sheet, reverse turn - answera: LEMARKHQ B: VICYFTW RT: PSDNG how many amino acids in alpha turn helix - answer3.6 Super secondary structure - answerhelix turn helix parallel vs. antiparallel - answerparallel wedges face same way antiparallel face opposite Isoelectric point (pI) - answerpH at which a particular molecule carries no net electrical charge. Protein Separation Techniques (ALL) - answerHomogenization Salting Out Affinity Chromatgraphy Ion Exchange Size Exclusion/Gel Filtration H, S, A, I, SG He said all individuals SinG Protein Separation Techniques (H) - answerHomogenization disrupt cells, sonicator, centrifuge Protein Separation Techniques (S) - answerSalting out different proteins precipitate out at different salt concentrations Protein Separation Techniques (A) - answerAffinity chromatography add a tag; histidine binds; see how well there is an affinity for the group Protein Separation Techniques (I) - answerion exchange separation based on charge; + proteins stick to - beads Protein Separation Techniques (SG) - answerSize Exclusion/Gel Filtration separation based on size Cuts (protein separation techniques) - answercut out what dont need/isnt protein (use 40% concen. then keep liquid; if use 60% concen. then keep pellet) Dialysis - answerprotein separation based on bad with pores 1. Moles in bag + moles in buffer 2. Divide moles by total volume - new concentration Electrophoresis - answergels - move by size with electric current 1. Isoelectric focusing - purely pH 2. SDS page pH separation is by pI and charge in 2d Protein sequencing (who cuts after what) - answerCyanogen bromide = methionine trypsin = K, and R chymotrypsin = F, M, L, W, Y IP - answerImmunoprecipitation with proteins 1. add antibodies specific to protein of interest to lysed cells 2. add antibody binding protein on beads 3. centrifuge and protein is on bead wi